Ingolia T D, Craig E A, McCarthy B J
Cell. 1980 Oct;21(3):669-79. doi: 10.1016/0092-8674(80)90430-4.
The primary sequence of the major heat shock gene of D. melanogaster, that for the 70,000 protein, has been determined. One of the reading frames is devoid of stop codons for over 2000 bp. The region between the first ATG and the first stop codon encodes a protein of molecular weight 70,270. The 5' end of the messenger RNA was localized in the DNA sequence by two independent methods. The 5' flanking sequences of three distinct 70K genes were also determined. Extensive homology in the primary sequences extends about 500 bp upstream from the ATG, which is the presumptive initiation of protein synthesis. Each 70K gene has the putative promoter sequence TATAAATA about 325 bp upstream from this ATG. A heptanucleotide sequence identified as the capping site for other messengers is found 24-30 bp downstream from the ends of the A-T-rich sequence. A 12 bp sequence with dyad symmetry begins 23 bp upstream from the beginning of the above A-T-rich sequence.
黑腹果蝇主要热休克基因（即编码70000蛋白的基因）的一级序列已被确定。其中一个阅读框在超过2000个碱基对的长度内没有终止密码子。第一个甲硫氨酸（ATG）与第一个终止密码子之间的区域编码一种分子量为70270的蛋白质。信使核糖核酸（mRNA）的5'端通过两种独立方法在DNA序列中定位。三个不同的70K基因的5'侧翼序列也已确定。一级序列中的广泛同源性从甲硫氨酸（这是假定的蛋白质合成起始位点）向上游延伸约500个碱基对。每个70K基因在该甲硫氨酸上游约325个碱基对处具有假定的启动子序列TATAAATA。一个被确定为其他信使RNA加帽位点的七核苷酸序列在富含A-T序列末端下游24 - 30个碱基对处被发现。一个具有二重对称的12个碱基对序列在上述富含A-T序列起始点上游23个碱基对处开始。
