Institute of Marine Biochemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
Arch Pharm Res. 2012 Dec;35(12):2135-42. doi: 10.1007/s12272-012-1210-8. Epub 2012 Dec 21.
Bioassay-guided fractionation based on the anti-inflammatory activity of a methanol extract of Ficus microcarpa leaves led to the isolation of seven galactolipids: 2(S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D-galactopyranoside (1), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-O-β-D-galactopyranoside (2), (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-O-β-D-galactopyranoside (3), (2S)-3-O-octadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (4), (2S)-2,3-O-dioctadeca-9Z,12Z,15Z-trienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (5), gingerglycolipid B (6), and (2S)-2,3-O-dioctadeca-9Z,12Z-dienoylglyceryl-6'-O-(α-D-galactopyranosyl)-β-D-galactopyranoside (7). Their chemical structures were elucidated by mass, 1D-, and 2D-NMR spectroscopic methods as well as chemical methods. The antiinflammatory effect of these compounds on TNF-α induced IL-8 secretion in the HT-29 cell line was evaluated. All above galactolipids showed significant inhibition ranging 40% at a concentration of 50 μM. The results suggest that galactolipids from the leaves of F. microcarpa may be used as potent anti-inflammatory agents.
基于 Ficus microcarpa 叶甲醇提取物的抗炎活性进行的生物测定指导分离，得到了七种半乳糖脂：2(S)-3-O-十八烷-9Z,12Z,15Z-三烯酰基甘油-O-β-D-半乳糖苷（1）、(2S)-2,3-O-十八烷-9Z,12Z,15Z-三烯酰基甘油-O-β-D-半乳糖苷（2）、(2S)-2,3-O-十八烷-9Z,12Z-二烯酰基甘油-O-β-D-半乳糖苷（3）、(2S)-3-O-十八烷-9Z,12Z,15Z-三烯酰基甘油-6'-O-(α-D-半乳糖吡喃基)-β-D-半乳糖苷（4）、(2S)-2,3-O-十八烷-9Z,12Z,15Z-三烯酰基甘油-6'-O-(α-D-半乳糖吡喃基)-β-D-半乳糖苷（5）、gingerglycolipid B（6）和(2S)-2,3-O-十八烷-9Z,12Z-二烯酰基甘油-6'-O-(α-D-半乳糖吡喃基)-β-D-半乳糖苷（7）。它们的化学结构通过质谱、1D 和 2D-NMR 光谱以及化学方法确定。评估了这些化合物对 HT-29 细胞系中 TNF-α 诱导的 IL-8 分泌的抗炎作用。所有上述半乳糖脂在 50 μM 浓度下均表现出显著的抑制作用，抑制率在 40%左右。结果表明，F. microcarpa 叶中的半乳糖脂可能可作为有效的抗炎剂。
Zotero 插件