Université de Rouen, IRCOF, Mont-Saint-Aignan, France.
J Am Soc Mass Spectrom. 2013 Feb;24(2):238-48. doi: 10.1007/s13361-012-0527-3. Epub 2012 Dec 21.
We reported the use of ion mobility (IM) combined with mass spectrometry (MS) as an analytical tool to investigate low generation polyamidoanine (PAMAM) dendrimers. This analytical approach has been employed to separate ions of defective structures with different charge state but exactly the same m/z value. Tandem mass spectrometry (MS/MS) after IM separation allowed a comprehensive structural characterization of defective dendrimers. In addition, IM was used to evaluate the collision cross-sections of ions of perfect dendrimers. They showed a good correlation with calculated collision cross-sections obtained by the trajectory method (TM) and were also consistent with dimensions reported by other established analytical methods.
我们报告了使用离子淌度（IM）与质谱（MS）相结合作为分析工具来研究低代聚酰胺-胺（PAMAM）树状大分子。这种分析方法已被用于分离具有不同电荷状态但具有相同质荷比的缺陷结构的离子。IM 分离后的串联质谱（MS/MS）允许对缺陷树突状大分子进行全面的结构表征。此外，IM 还用于评估完美树突状大分子离子的碰撞截面。它们与通过轨迹方法（TM）获得的计算碰撞截面很好地相关，并且与其他已建立的分析方法报告的尺寸也一致。
