Environmental Biology Division, National Institute for Environmental Studies (NIES), Tsukuba, Ibaraki 305-8506, Japan.
Biotechnol Lett. 2013 Apr;35(4):647-56. doi: 10.1007/s10529-012-1118-x. Epub 2012 Dec 22.
The 'ozone (O3)-responsive transcriptome' behavior in the panicles and grains of rice plant was studied individually through high-throughput oligo-DNA microarray technique. O3 differentially and separately regulated 620 and 130 genes in the panicles and grains. Among the O3-responsive genes, 176 and 444 genes were up- and down-regulated in panicle compared to 24 and 106 genes in grain, respectively. Further mapping revealed that the majority of differentially expressed genes were mainly involved in signaling, hormonal, cell wall, transcription, proteolysis, and defense events. Many previously unknown O3-responsive novel genes were identified. Inventory of 745 O3-responsive genes and their mapping will expand our knowledge on novel regulatory processes in both panicles and grains of rice; and, serve as a resource towards the designing of rice crops for future high-O3world.
Tropospheric ozone (O3) severely affects agricultural production worldwide. Present study aims to reveal a detailed O3 responsive gene network in panicle and grains of rice plants through transcriptomics approach. Our results provide an insight into the basis of O3-response in rice plants, and will help to develop suitable rice genotype for future high O3- world.
通过高通量寡核苷酸 DNA 微阵列技术，分别对水稻穗和籽粒中的“臭氧（O3）响应转录组”行为进行了研究。O3 分别在穗和籽粒中差异调控了 620 个和 130 个基因。在 O3 响应基因中，与籽粒相比，穗中有 176 个和 444 个基因分别上调和下调，而籽粒中分别有 24 个和 106 个基因上调和下调。进一步的基因图谱分析表明，大部分差异表达基因主要参与信号转导、激素、细胞壁、转录、蛋白水解和防御反应等事件。鉴定出了许多以前未知的 O3 响应新基因。745 个 O3 响应基因及其图谱的编制将扩展我们对水稻穗和籽粒中新型调控过程的认识，并为未来高 O3 世界设计水稻作物提供资源。
对流层臭氧（O3）严重影响全球农业生产。本研究旨在通过转录组学方法揭示水稻穗和籽粒中 O3 响应的详细基因网络。我们的研究结果深入了解了水稻植物对 O3 的响应基础，并将有助于开发适合未来高 O3 世界的水稻基因型。
