Laboratory of Plant Epigenetics and Evolution, School of Life Sciences, Liaoning University, Shenyang 110036, China.
Academy of Agricultural and Forestry Sciences, Qinghai University, Xining 810016, China.
Genes (Basel). 2023 Sep 28;14(10):1888. doi: 10.3390/genes14101888.
With the development of urban industrialization, the increasing ozone concentration (O) at ground level stresses on the survival of plants. Plants have to adapt to ozone stress. DNA methylation is crucial for a rapid response to abiotic stress in plants. Little information is known regarding the epigenetic response of DNA methylation of plants to O stress. This study is designed to explore the epigenetic mechanism and identify a possible core modification of DNA methylation or genes in the plant, in response to O stress. We investigated the agronomic traits and genome-wide DNA methylation variations of the Japonica rice cultivar Nipponbare in response to O stress at three high concentrations (80, 160, and 200 nmol·mol), simulated using open-top chambers (OTC). The flag leaf length, panicle length, and hundred-grain weight of rice showed beneficial effects at 80 nmol·mol O and an inhibitory effect at both 160 and 200 nmol·mol O The methylation-sensitive amplified polymorphism results showed that the O-induced genome-wide methylation alterations account for 14.72-15.18% at three different concentrations. Our results demonstrated that methylation and demethylation alteration sites were activated throughout the O stress, mainly at CNG sites. By recovering and sequencing bands with methylation alteration, ten stress-related differentially amplified sequences, widely present on different chromosomes, were obtained. Our findings show that DNA methylation may be an active and rapid epigenetic response to ozone stress. These results can provide us with a theoretical basis and a reference to look for more hereditary information about the molecular mechanism of plant resistance to O pollution.
随着城市化工业化的发展，地面臭氧浓度（O）的增加给植物的生存带来了压力。植物必须适应臭氧胁迫。DNA 甲基化对于植物对非生物胁迫的快速反应至关重要。然而，关于植物对 O 胁迫的 DNA 甲基化的表观遗传反应的信息知之甚少。本研究旨在探索表观遗传机制，并确定植物对 O 胁迫响应的 DNA 甲基化或基因的可能核心修饰。我们研究了在使用开顶式气室（OTC）模拟的三种高浓度（80、160 和 200nmol·mol）下，粳稻品种日本晴对 O 胁迫的农艺性状和全基因组 DNA 甲基化变化。水稻的旗叶长度、穗长和百粒重在 80nmol·mol O 下表现出有益的影响，而在 160 和 200nmol·mol O 下表现出抑制作用。甲基化敏感扩增多态性结果表明，O 诱导的全基因组甲基化改变占三个不同浓度的 14.72-15.18%。我们的结果表明，在整个 O 胁迫过程中，甲基化和去甲基化改变位点被激活，主要发生在 CNG 位点。通过回收和测序具有甲基化改变的条带，获得了十个与胁迫相关的差异扩增序列，广泛存在于不同染色体上。我们的发现表明，DNA 甲基化可能是对臭氧胁迫的一种活跃而快速的表观遗传反应。这些结果可以为我们提供理论基础和参考，以寻找更多关于植物对 O 污染抗性的分子机制的遗传信息。
