Department of Food Science and Biotechnology, National Chung Hsing University, 250 Kuo-Kuang Rd., Taichung 402, Taiwan, ROC.
Food Chem Toxicol. 2013 Mar;53:376-83. doi: 10.1016/j.fct.2012.12.018. Epub 2012 Dec 22.
Infection with Helicobacter pylori causes extensive gastric epithelial cell inflammation which may progress to atrophic gastritis, intestinal metaplasia, and even gastric adenocarcinoma. Apigenin (4',5,7-trihydroxyflavone) is widely distributed in fruits and vegetables, and is a well-known antiinflammatory supplement with low cytotoxicity. In this study, we investigated the anti-inflammatory effects of apigenin in H. pylori-infected MKN45 cells, for which IκBα, cyclooxygenase-2 (COX-2), intercellular adhesion molecule-1 (ICAM-1), reactive oxygen species (ROS), interleukin-8 (IL-8), IL-6, IL-1β, and mucin-2 (MUC-2) expressions were examined. Apigenin treatments (9.3-74 μM) significantly increased the IκBα expression, and thus inhibited nuclear factor kappa B (NF-κB) activation, and the inflammatory factor (COX-2, ICAM-1, ROS, IL-6, and IL-8) expressions decreased. The ROS levels decreased partially based on the intrinsic scavenging property of apigenin. In summary, apigenin treatments effectively inhibited NF-κB activation and the related inflammatory factor expressions, as well as increased MUC-2 expression in the H. pylori-infected MKN45 cells. The compound shows great potential as a candidate agent for the inhibition of H. pylori-induced extensive gastric epithelial cell inflammation.
幽门螺杆菌感染会引起广泛的胃上皮细胞炎症，可能进展为萎缩性胃炎、肠上皮化生，甚至胃腺癌。芹菜素（4'，5，7-三羟基黄酮）广泛存在于水果和蔬菜中，是一种具有低细胞毒性的知名抗炎补充剂。在这项研究中，我们研究了芹菜素在幽门螺杆菌感染的 MKN45 细胞中的抗炎作用，检测了 IκBα、环氧化酶-2（COX-2）、细胞间黏附分子-1（ICAM-1）、活性氧（ROS）、白细胞介素-8（IL-8）、白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）和粘蛋白-2（MUC-2）的表达。芹菜素处理（9.3-74 μM）显著增加了 IκBα 的表达，从而抑制了核因子 kappa B（NF-κB）的激活，降低了炎症因子（COX-2、ICAM-1、ROS、IL-6 和 IL-8）的表达。ROS 水平的降低部分基于芹菜素的内在清除特性。综上所述，芹菜素处理可有效抑制 NF-κB 激活和相关炎症因子的表达，同时增加幽门螺杆菌感染的 MKN45 细胞中 MUC-2 的表达。该化合物作为抑制幽门螺杆菌诱导的广泛胃上皮细胞炎症的候选药物具有很大的潜力。
