Department of Microbiology, Gyeongsang National University School of Medicine, Gyeongsang Institute of Health Science, Jinju 660-751, South Korea.
Microbiol Immunol. 2013 May;57(5):366-73. doi: 10.1111/1348-0421.12049.
Infection with Helicobacter pylori leads to gastritis, peptic ulcers and gastric cancer. Moreover, when the gastric mucosa is exposed to H. pylori, gastric mucosal inflammatory cytokine interleukin-8 (Il-8) and reactive oxygen species increase. Anthocyanins have anti-oxidative, antibacterial and anti-inflammatory properties. However, the effect of anthocyanins in H. pylori-infected cells is not yet clear. In this study, therefore, the effect of anthocyanins on H. pylori-infected human gastric epithelial cells was examined. AGS cells were pretreated with anthocyanins for 24 hrs followed by H. pylori 26695 infection for up to 24 hrs. Cell viability and ROS production were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and 2',7'-dichlorofluorescein diacetate assay, respectively. Western blot analyses and RT-PCR were performed to assess gene and protein expression, respectively. IL-8 secretion in AGS cells was measured by ELISA. It was found that anthocyanins decrease H. pylori-induced ROS enhancement. Anthocyanins also inhibited phosphorylation of mitogen-activated protein kinases, translocation of nuclear factor-kappa B and Iκβα degradation. Furthermore anthocyanins inhibited H. pylori-induced inducible nitric oxide synthases and cyclooxygenase-2 mRNA expression and inhibited IL-8 production by 45.8%. Based on the above findings, anthocyanins might have an anti-inflammatory effect in H. pylori-infected gastric epithelial cells.
幽门螺杆菌感染可导致胃炎、消化性溃疡和胃癌。此外，当胃黏膜暴露于幽门螺杆菌时，胃黏膜炎症细胞因子白细胞介素-8（IL-8）和活性氧增加。花色苷具有抗氧化、抗菌和抗炎作用。然而，花色苷在幽门螺杆菌感染细胞中的作用尚不清楚。因此，本研究旨在研究花色苷对幽门螺杆菌感染的人胃上皮细胞的影响。AGS 细胞用花色苷预处理 24 小时，然后用幽门螺杆菌 26695 感染长达 24 小时。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴盐和 2',7'-二氯荧光素二乙酸酯测定法分别检测细胞活力和 ROS 产生。通过 Western blot 分析和 RT-PCR 分别评估基因和蛋白质表达。通过 ELISA 测量 AGS 细胞中 IL-8 的分泌。结果发现，花色苷可降低幽门螺杆菌诱导的 ROS 增强。花色苷还抑制丝裂原活化蛋白激酶的磷酸化、核因子-κB 的易位和 Iκβα 的降解。此外，花色苷抑制幽门螺杆菌诱导的诱导型一氧化氮合酶和环氧化酶-2 mRNA 表达，并抑制 IL-8 产生 45.8%。基于上述发现，花色苷可能对幽门螺杆菌感染的胃上皮细胞具有抗炎作用。
