Rombouts Charlotte, Jeanneau Charlotte, Bakopoulou Athina, About Imad
Aix Marseille Université, CNRS, ISM UMR 7287, 13288, Marseille, France.
Department of Fixed Prosthesis & Implant Prosthodontics, School of Dentistry, Aristotle University of Thessaloniki, GR-54124, Thessaloniki, Greece.
Dent J (Basel). 2016 Mar 25;4(2):8. doi: 10.3390/dj4020008.
The recruitment of dental pulp stem cells (DPSC) is a prerequisite for the regeneration of dentin damaged by severe caries and/or mechanical injury. Understanding the complex process of DPSC recruitment will benefit future tissue engineering applications based on the stimulation of endogenous DPSC for dentin pulp regeneration. The current known mobilization signals and subsequent migration of DPSC towards the lesion site, which is influenced by the pulp inflammatory state and the application of pulp capping materials, are reviewed. The research outcome of migration studies may be affected by the applied methodology, which should thus be chosen with care. Both the advantages and disadvantages of commonly used assays for investigating DPSC migration are discussed. This review highlights the fact that DPSC recruitment is dependent not only on the soluble chemotactic signals, but also on their interaction with neighboring cells and the extracellular matrix, which can be modified under pathological conditions. These are discussed to explain how these modifications lead to the stimulation of DPSC recruitment.
招募牙髓干细胞（DPSC）是严重龋齿和/或机械损伤所致牙本质再生的前提条件。了解DPSC招募的复杂过程将有助于未来基于刺激内源性DPSC进行牙髓牙本质再生的组织工程应用。本文综述了目前已知的DPSC动员信号以及随后DPSC向病变部位的迁移，这一过程受牙髓炎症状态和盖髓材料应用的影响。迁移研究的结果可能会受到所应用方法的影响，因此应谨慎选择方法。本文讨论了用于研究DPSC迁移的常用检测方法的优缺点。本综述强调了这样一个事实，即DPSC招募不仅依赖于可溶性趋化信号，还依赖于它们与邻近细胞和细胞外基质的相互作用，而这种相互作用在病理条件下可能会发生改变。本文对这些内容进行了讨论，以解释这些改变如何导致DPSC招募受到刺激。
