Chou M Y, Chang A L, McBride J, Donoff B, Gallagher G T, Wong D T
Department of Oral Medicine and Oral Pathology, Harvard School of Dental Medicine, Boston, Massachusetts 02115.
Am J Pathol. 1990 Apr;136(4):729-33.
A general method applicable for the determination of any mammalian tissue's proliferative pattern is described. This method determines the cellular mRNA level of a proliferation-dependent gene, histone H3, by in situ hybridization. The cell-cycle S-phase-specific expression of this highly conserved ubiquitous cellular gene, and the lack of it in resting cells, permits the unambiguous identification of cycling cells in any tissues, normal or diseased. This method can be conveniently coupled with routine biopsy and could be streamlined for a central laboratory with results obtainable in 2 days. Furthermore, this procedure works successfully on formalin-fixed paraffin-embedded sections, thus allowing retrospective studies of biopsies or autopsy materials.
本文描述了一种适用于确定任何哺乳动物组织增殖模式的通用方法。该方法通过原位杂交测定增殖相关基因组蛋白H3的细胞mRNA水平。这种高度保守的普遍存在的细胞基因在细胞周期S期特异性表达，而在静止细胞中不表达，这使得能够明确鉴定任何正常或患病组织中的增殖细胞。该方法可以方便地与常规活检相结合，并且可以为中心实验室进行简化，2天即可获得结果。此外，该程序在福尔马林固定石蜡包埋切片上成功运行，从而允许对活检或尸检材料进行回顾性研究。
