Department of Laboratory Medicine, The 82nd Hospital of the People's Liberation Army, No.100 Jiankang East Road, Huaian 223001, China.
Mol Biol Rep. 2013 May;40(5):3577-82. doi: 10.1007/s11033-012-2431-0. Epub 2012 Dec 30.
MicroRNAs (miRNAs) are short non-coding RNAs that are involved in post-transcriptional regulation of gene expression. Hsa-miR-26b is an intronic miRNA located in the intron of CTDSP1 (carboxy-terminal domain, RNA polymerase II, polypeptide A, small phosphatase 1). In the present study, the expression of hsa-miR-26b was examined during human preadipocyte differentiation. 15 days after induction of differentiation, mature human adipocytes were treated with adipokines. Hsa-miR-26b was differentially expressed during human preadipocyte differentiation. Tumor necrosis factor-α (TNF-α), leptin and resistin, but not interleukin-6, caused downregulation of hsa-miR-26b expression in adipocytes. These results suggest that the expression of hsa-miR-26b is affected by TNF-α, leptin and resistin and that hsa-miR-26b may be an important mediator in regulating the obesity-related insulin sensitivity and inflammatory responses.
微小 RNA（miRNA）是参与基因表达转录后调控的短非编码 RNA。hsa-miR-26b 是一种位于 CTDSP1（羧基末端结构域，RNA 聚合酶 II，多肽 A，小磷酸酶 1）内含子中的内含子 miRNA。本研究检测了 hsa-miR-26b 在人前脂肪细胞分化过程中的表达情况。诱导分化 15 天后，用脂肪细胞因子处理成熟的人脂肪细胞。hsa-miR-26b 在人前脂肪细胞分化过程中差异表达。肿瘤坏死因子-α（TNF-α）、瘦素和抵抗素，但不是白细胞介素-6，导致脂肪细胞中 hsa-miR-26b 表达下调。这些结果表明，hsa-miR-26b 的表达受 TNF-α、瘦素和抵抗素的影响，hsa-miR-26b 可能是调节肥胖相关胰岛素敏感性和炎症反应的重要介质。
