Key Laboratory of Sichuan Province for Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Exploitation, Chengdu, 610041, China.
Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Ministry of Education, Southwest Minzu University, Chengdu, 610041, China.
In Vitro Cell Dev Biol Anim. 2021 Mar;57(3):257-263. doi: 10.1007/s11626-020-00493-y. Epub 2021 Jan 28.
MicroRNAs are a class of highly conserved and widely distributed non-coding RNAs. It is known that miR-26b has a high abundance in adipose tissue and is considered to be an effective regulator of adipogenesis. However, it is unclear whether miR-26b-5p, the product of miR-26b precursor, has the same effect as miR-26b. In the present study, we explored the potential role of miR-26b-5p in preadipocyte differentiation of goats. We found that the expression of miR-26b-5p had dramatic change during goat intramuscular preadipocyte differentiation. Transfection and RT-qPCR revealed that overexpression of miR-26b-5p increased the level of adipogenic marker genes and lipid accumulation in goat preadipocyte, suggesting that miR-26b-5p positively regulates goat preadipocyte differentiation. Furthermore, bioinformatics analysis and dual fluorescein reporter assays were performed to predict and validate the targets of miR-26b-5p. The results showed that miR-26b-5p has a binding site in the 3'UTR of FGF21 and overexpression of miR-26b-5p significantly down-regulated the expression of FGF21 mRNA. Luciferase activity assays confirmed that miR-26b-5p is a positive regulator of goat intramuscular preadipocyte via targeting FGF21. These findings provide reference for further revealing of the regulatory networks of goat fat metabolism and contribute to a better understanding of intramuscular fat deposition in goats.
微小 RNA 是一类高度保守且广泛分布的非编码 RNA。已知 miR-26b 在脂肪组织中丰度较高，被认为是脂肪生成的有效调节因子。然而，miR-26b-5p 是否具有与 miR-26b 相同的作用尚不清楚，miR-26b-5p 是 miR-26b 前体的产物。在本研究中，我们探讨了 miR-26b-5p 在山羊肌前体细胞分化中的潜在作用。我们发现 miR-26b-5p 的表达在山羊肌前体细胞分化过程中发生了显著变化。转染和 RT-qPCR 结果表明，miR-26b-5p 的过表达增加了山羊前体脂肪细胞中成脂标记基因的水平和脂质积累，表明 miR-26b-5p 正向调节山羊前体脂肪细胞分化。此外，进行了生物信息学分析和双荧光素酶报告基因实验，以预测和验证 miR-26b-5p 的靶标。结果表明，miR-26b-5p 在 FGF21 的 3'UTR 上具有结合位点，miR-26b-5p 的过表达显著下调了 FGF21 mRNA 的表达。荧光素酶活性测定证实 miR-26b-5p 通过靶向 FGF21 正向调控山羊肌前体脂肪细胞。这些发现为进一步揭示山羊脂肪代谢的调控网络提供了参考，并有助于更好地理解山羊肌内脂肪沉积。
