牛病毒性腹泻病毒NS3蛋白B细胞线性表位的定位
Mapping B-cell linear epitopes of NS3 protein of bovine viral diarrhea virus.
作者信息
Li Yan, Jia Ying, Wen Kai, Liu Hua, Gao Mingchun, Ma Bo, Zhang Wenlong, Wang Junwei
机构信息
College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, PR China.
出版信息
Vet Immunol Immunopathol. 2013 Feb 15;151(3-4):331-6. doi: 10.1016/j.vetimm.2012.11.011. Epub 2012 Dec 10.
Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. NS3 is one of the immunodominance regions of the BVDV viral proteins. To identify the potential B-cell linear antigenic epitopes within BVDV NS3 region, serial overlapping truncations covering the whole region were expressed and purified, and screened by multistep of Western-blot. We found ((1)VCKKITEHERCHVNI(15)), ((20)AFFGVMPRGTTPRAPVR(36)), ((46)RRGLETGWAYTHQGGI(61)), ((281)EGDMATGITYASYGYFC(297)), ((426)YSGEDPANLRVVTSQSPYVVVATNAIESGV(455)) and ((481)FIVTGLKRMAVTVGEQA(497)) can be recognized by the BVDV infected bovine serum. These proteins have been confirmed by indirect enzyme-linked immunosorbent assay (I-ELISA). The results of this study might open new perspectives on the structure and antibody-antigen reaction of the non-structural proteins and may aid in the clinical application as well.
牛病毒性腹泻病毒（BVDV）是黄病毒科瘟病毒属的成员。NS3是BVDV病毒蛋白的免疫显性区域之一。为了鉴定BVDV NS3区域内潜在的B细胞线性抗原表位，表达并纯化了覆盖整个区域的一系列重叠截短片段，并通过多步蛋白质免疫印迹法进行筛选。我们发现（（1）VCKKITEHERCHVNI（15））、（（20）AFFGVMPRGTTPRAPVR（36））、（（46）RRGLETGWAYTHQGGI（61））、（（281）EGDMATGITYASYGYFC（297））、（（426）YSGEDPANLRVVTSQSPYVVVATNAIESGV（455））和（（481）FIVTGLKRMAVTVGEQA（497））能够被感染BVDV的牛血清识别。这些蛋白已通过间接酶联免疫吸附测定（I-ELISA）得到证实。本研究结果可能为非结构蛋白的结构及抗体-抗原反应开辟新的视角，也可能有助于临床应用。
Zotero 插件