Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Londrina, PR, Brazil.
Vet Res Commun. 2013 Jun;37(2):183-6. doi: 10.1007/s11259-012-9548-4. Epub 2013 Jan 4.
The aims of this study were to evaluate torque teno sus virus (TTSuV) infection in healthy slaughter-age pigs and to compare the similarities of the untranslated region (UTR) nucleotide (nt) sequences obtained from different biological samples of the same animals. Fifty-eight pigs were evaluated by PCR assay for the presence of TTSuV in paired samples of liver and serum (Group 1, n = 27) and lung and serum (Group 2, n = 31). All the pigs were positive for TTSuV infection in the organs sampled and 94.8 % (n = 55) presented with viraemia. The nt sequence similarities between the Groups 1 and 2 varied from 91.7 % to 96.6 % (TTSuV1) and 91 % to 95 % (TTSuV2). In Group 1, the nt sequence similarities were 93 % (TTSuV1) and 95.4 % (TTSuV2). In Group 2, the nt sequence similarities were 95 % (TTSuV1) and 91 % (TTSuV2). These results revealed the simultaneous infection with distinct strains of TTSuV1 and 2 in healthy pigs at slaughter age.
本研究旨在评估屠宰年龄健康猪中扭矩藤病毒（TTSuV）的感染情况，并比较同一动物不同生物样本中获得的非翻译区（UTR）核苷酸（nt）序列的相似性。将 58 头猪进行 PCR 检测，以评估其肝脏和血清（第 1 组，n = 27）和肺脏和血清（第 2 组，n = 31）配对样本中 TTSuV 的存在情况。所有采样器官均呈 TTSuV 感染阳性，94.8%（n = 55）呈病毒血症。第 1 组和第 2 组之间的 nt 序列相似性从 91.7%到 96.6%（TTSuV1）和 91%到 95%（TTSuV2）不等。第 1 组的 nt 序列相似性为 93%（TTSuV1）和 95.4%（TTSuV2）。第 2 组的 nt 序列相似性为 95%（TTSuV1）和 91%（TTSuV2）。这些结果表明，在屠宰年龄的健康猪中同时存在不同株 TTSuV1 和 2 的感染。
