双环糊精体系在毛细管电泳对映体分离中的应用。

Application of Dual-Cyclodextrin Systems in Capillary Electrophoresis Enantioseparations.

作者信息

Servais Anne-Catherine, Fillet Marianne

机构信息

Laboratory for the Analysis of Medicines, Department of Pharmaceutical Sciences, CIRM, Quartier Hôpital, University of Liège, Liège, Belgium.

出版信息

Methods Mol Biol. 2019;1985:357-364. doi: 10.1007/978-1-4939-9438-0_19.

DOI:10.1007/978-1-4939-9438-0_19

PMID:31069744

Abstract

The enantioseparation of acidic and neutral compounds can be successfully achieved in capillary electrophoresis (CE) using dual-cyclodextrin (CD) systems. This chapter describes how to separate the enantiomers of acidic or neutral substances using dual-CD systems made up of a negatively charged CD derivative, i.e., sulfobutyl-β-CD or carboxymethyl-β-CD, in combination with a neutral one, namely heptakis(2,3,6-tri-O-methyl)-β-CD. An acidic compound (carprofen) and a weakly acidic drug (pentobarbital) were selected as model compounds.

摘要

使用双环糊精（CD）体系，可在毛细管电泳（CE）中成功实现酸性和中性化合物的对映体分离。本章介绍了如何使用由带负电荷的CD衍生物（即磺丁基-β-环糊精或羧甲基-β-环糊精）与中性环糊精（即七（2,3,6-三-O-甲基）-β-环糊精）组成的双CD体系来分离酸性或中性物质的对映体。选择一种酸性化合物（卡洛芬）和一种弱酸性药物（戊巴比妥）作为模型化合物。