Beijing Advanced Innovation Center for Food Nutrition and Human Health, Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 10083, China.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Nutrients. 2020 Apr 17;12(4):1130. doi: 10.3390/nu12041130.
Chondroitin sulfate (CS), mainly present in the cartilage and bone of animals, is known as a potential food-derived bioactive that has several biological functions, such as anti-arthritic and anti-inflammatory activity. Sturgeon (), an important fishery resource in China, contains an abundance of CS in their cartilage. In our previous study, we have extracted and purified CS from sturgeon cartilage. Herein, we further investigate the health benefits of sturgeon-derived chondroitin sulfate (SCS), especially for colorectal cancer treatment. The in vitro study indicated that SCS could inhibit the proliferation of the human colon cancer cell line HCT-116 in a dose-dependent manner, which was associated with cell cycle arrest. In addition, SCS also led to extensive cellular apoptosis in colon cancer cell HCT-116 cells. Meanwhile, an in vivo study showed that SCS treatment significantly inhibited the tumor development of xenograft HCT-116 in mice via proliferation suppression and apoptosis induction. Further, a mechanistic study demonstrated that the apoptosis induction was mainly due to the activation of the family-associated mitochondrial pathway. Overall, our results provided a basis for SCS as a promising agent against colon cancer.
硫酸软骨素（CS）主要存在于动物的软骨和骨骼中，是一种具有多种生物学功能的潜在的食物源生物活性物质，具有抗关节炎和抗炎活性。鲟鱼（）是中国重要的渔业资源，其软骨中含有丰富的 CS。在我们之前的研究中，我们已经从鲟鱼软骨中提取和纯化了 CS。在此，我们进一步研究了鲟鱼来源的硫酸软骨素（SCS）对健康的益处，特别是在治疗结直肠癌方面。体外研究表明，SCS 可以剂量依赖性地抑制人结肠癌细胞系 HCT-116 的增殖，这与细胞周期阻滞有关。此外，SCS 还导致结肠癌细胞 HCT-116 细胞广泛发生细胞凋亡。同时，体内研究表明，SCS 治疗通过抑制增殖和诱导凋亡，显著抑制了异种移植 HCT-116 小鼠的肿瘤发展。此外，机制研究表明，凋亡诱导主要归因于家族相关的线粒体途径的激活。总之，我们的研究结果为 SCS 作为一种有前途的结肠癌治疗药物提供了依据。
