de Camargo Ronaldo Antônio, da Costa Ellen Dias, Catisti Rosana
Fundação Hermínio Ometto, Uniararas, Araras, SP, Brazil.
Homeopathy. 2013 Jan;102(1):49-53. doi: 10.1016/j.homp.2012.11.001.
To analyze the effect of homeopathic Arnica on mitochondrial oxidative stress induced by Ca(2+) plus inorganic phosphate and/or Fe(2+)-citrate-mediated lipid peroxidation through changes in oxygen consumption rates.
Mitochondria were isolated by differential centrifugation from the livers of adult male Wistar rats which had been treated with Arnica montana 6cH, 12cH, 30cH or succussed 30% ethanol (control) for 21 days.
In the presence of antimycin-A, electron transport chain inhibitor, as evidenced by antimycin-A insensitive O(2) consumption, Arnica inhibited lipid peroxidation of mitochondrial membranes. In oxidative stress conditions, in the presence of Ca(2+) and inorganic phosphate, animals receiving Arnica 30cH had a significant decrease in mitochondrial O(2) consumption compared to control animals.
When administrated orally, Arnica 30cH protects against hepatic mitochondrial membrane permeabilization induced by Ca(2+) and/or Fe(2+)-citrate-mediated lipid peroxidation and fragmentation of proteins due to the attack by reactive oxygen species.
通过分析氧消耗率的变化，研究顺势疗法山金车对由钙离子加无机磷酸盐和/或柠檬酸亚铁介导的脂质过氧化所诱导的线粒体氧化应激的影响。
通过差速离心法从成年雄性Wistar大鼠肝脏中分离出线粒体，这些大鼠已用6cH、12cH、30cH山金车或30%乙醇震荡处理（对照）21天。
在存在抗霉素A（电子传递链抑制剂）的情况下，抗霉素A不敏感的氧消耗证明，山金车抑制线粒体膜的脂质过氧化。在氧化应激条件下，在存在钙离子和无机磷酸盐的情况下，与对照动物相比，接受30cH山金车的动物线粒体氧消耗显著降低。
口服30cH山金车可保护肝脏线粒体膜免受由钙离子和/或柠檬酸亚铁介导的脂质过氧化以及活性氧攻击导致的蛋白质片段化所引起的通透性改变。
