BIOTEC Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Thailand Science Park, Phaholyothin Road, Klong 1, Klong Luang, Pathumthani 12120, Thailand.
Biological Resource Center, National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba, 292-0812, Japan.
Int J Syst Evol Microbiol. 2013 Jul;63(Pt 7):2649-2654. doi: 10.1099/ijs.0.046946-0. Epub 2013 Jan 4.
A novel actinomycete strain, A-T 4600(T), which developed cylindrical sporangia containing a longitudinal pair of motile spores forming singly or in bundles on short ramifications of the aerial mycelium, was isolated from soil collected from an evergreen forest in Thailand. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained ribose, madurose, mannose and glucose. The predominant menaquinones were MK-9(H2). Mycolic acids were not detected. The diagnostic phospholipids were phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannoside, diphosphatidylglycerol and aminophosphoglycolipid. The predominant cellular fatty acids were unsaturated C17 : 1, C18 : 1, saturated C16 : 0, and C17 : 0. The G+C content of the DNA was 70.8 mol%. Phenotypic and chemotaxonomic analyses showed that the isolate had the typical characteristics of members of the genus Planobispora. Furthermore, 16S rRNA gene sequence analysis also indicated that this strain belonged to the genus Planobispora but as a putative novel species. DNA-DNA relatedness values that differentiate the isolate from previously described members of the genus Planobispora were significantly below 70 %. Following an evaluation of phenotypic, chemotaxonomic and genotypic studies, it is proposed that the isolate represents a novel species, Planobispora siamensis sp. nov.; the type strain is A-T 4600(T) ( = BCC 39469(T) = NBRC 107568(T)).
一株新型放线菌 A-T4600(T)，从泰国常绿森林土壤中分离得到，该菌气生菌丝短分支上形成单个或成束的纵向对生的圆柱状孢子囊，内含一对能运动的孢子。其细胞壁肽聚糖含有meso-二氨基庚二酸。全细胞糖含有核糖、甘露糖、葡萄糖和甘露糖。主要的menaquinone 为 MK-9(H2)。未检测到(myco)lic acids。特征性磷脂为磷脂酰乙醇胺、磷脂甲基乙醇胺、磷脂酰肌醇、磷脂酰甘油、磷脂酰肌醇甘露糖苷、双磷脂酰甘油和氨基磷脂酰甘油。主要的细胞脂肪酸为不饱和 C17:1、C18:1、饱和 C16:0 和 C17:0。DNA G+C 含量为 70.8 mol%。表型和化学分类分析表明，该分离株具有 Planobispora 属成员的典型特征。此外，16S rRNA 基因序列分析也表明该菌株属于 Planobispora 属，但属于一个假定的新种。该菌株与先前描述的 Planobispora 属成员的 DNA-DNA 同源性值明显低于 70%。经过对表型、化学分类和基因型研究的评估，建议该分离株代表一个新种，即暹罗拟无枝酸菌(Planobispora siamensis)sp. nov.；其模式菌株为 A-T4600(T)（=BCC 39469(T)=NBRC 107568(T)）。
