Poncelet S M, Limet J N, Noel J P, Kayaert M C, Galanti L, Collet-Cassart D
Unit of Experimental Medicine, Faculty of Medicine, Catholic University of Louvain, Brussels, Belgium.
J Immunoassay. 1990;11(1):77-88. doi: 10.1080/01971529008053259.
We set up an immunoassay by particle counting for theophylline. Theophylline concentration is assayed by its capacity to inhibit the agglutination of theophylline coated latex particles by a specific monoclonal antibody, the agglutination being enhanced by a rabbit anti-mouse IgG antiserum. The dose range is 2-64 mg/L. The cross-reactions observed with caffeine (0.3%), theobromine (0.2%), 3-methylxanthine (0.7%) and 8-chlorotheophylline (2%) are very good when compared with other published methods. Within and between-run precisions measured at low, medium and high level of the calibration curve show coefficients of variation ranging from 3.9% to 9.5%. Our assay was correlated with the Fluorescence Polarization Immunoassay (FPIA) and a correlation coefficient of 0.96 was determined for 89 samples.
我们建立了一种通过颗粒计数法检测茶碱的免疫测定法。茶碱浓度通过其抑制特定单克隆抗体对包被有茶碱的乳胶颗粒的凝集作用来测定，凝集作用可被兔抗小鼠IgG抗血清增强。剂量范围为2 - 64 mg/L。与其他已发表的方法相比，观察到与咖啡因（0.3%）、可可碱（0.2%）、3 - 甲基黄嘌呤（0.7%）和8 - 氯茶碱（2%）的交叉反应非常良好。在校准曲线的低、中、高浓度水平下测量的批内和批间精密度显示变异系数范围为3.9%至9.5%。我们的测定法与荧光偏振免疫测定法（FPIA）相关，对89个样本测定的相关系数为0.96。
