Behavioral Physiology of Farm Animals, Institute for Animals Husbandry and Breeding, University of Hohenheim, 70599 Stuttgart, Germany; Department of Animal Physiology, University of Bayreuth, 95447 Bayreuth, Germany.
Behavioral Physiology of Farm Animals, Institute for Animals Husbandry and Breeding, University of Hohenheim, 70599 Stuttgart, Germany.
Brain Behav Immun. 2013 Mar;29:156-165. doi: 10.1016/j.bbi.2012.12.019. Epub 2013 Jan 4.
The effects of social stress on several blood immune measures and collagen-induced arthritis (CIA) were investigated in Wistar rats using the resident-intruder confrontation paradigm to induce stress of different intensity. Male intruders were exposed for one week to a dominant opponent either repeatedly for 4h daily (moderate stress) or continuously (severe stress). Arthritis was induced by intradermal injection of collagen type II (CII) into the tail skin at the end of day 3 of confrontation. Only severe stress was associated with decreased CD4 and CD8 T cells, and the increase in granulocyte numbers and body mass loss was more pronounced under these conditions. Only severe stress reduced the susceptibility to arthritis by about 50%. Severity scores did not differ in the first five days after disease onset between all groups. Subsequent experiments focused on severely stressed rats indicated that disease progressed until day 10 only in control animals, but not in severely stressed males. Stressor exposure resulted in increased blood monocyte numbers, but these males failed to accumulate macrophages into the skin at the site of CII injection. High numbers of attacks experienced by intruders correlated with delayed disease onset in severely stressed rats. We hypothesize that severe stress persisting after disease induction exhibits beneficial effects on the susceptibility of CIA and propose that the specific endocrine and immunological profile associated with severe stress is an important factor for disease outcome--a factor which probably explains many of the conflicting data of previous stress studies on CIA.
采用“居民-入侵者对峙”模型，通过不同强度的压力源，研究社会压力对几种血液免疫指标和胶原诱导性关节炎（CIA）的影响，该模型用于诱导不同强度的压力。雄性入侵者连续一周每天暴露于优势对手 4 小时（中度压力）或连续暴露（重度压力）。在对峙第 3 天的尾巴皮肤皮内注射 II 型胶原（CII）以诱导关节炎。只有重度压力与 CD4 和 CD8 T 细胞减少有关，并且在这些条件下，粒细胞数量增加和体重减轻更为明显。只有重度压力使关节炎的易感性降低了约 50%。在发病后的前 5 天，所有组之间的严重程度评分均无差异。随后对重度应激大鼠的实验表明，在对照动物中疾病进展到第 10 天，但在重度应激雄性动物中则不会。应激源暴露导致血液单核细胞数量增加，但这些雄性动物未能将巨噬细胞积聚到 CII 注射部位的皮肤中。入侵者经历的高攻击次数与重度应激大鼠的疾病发病延迟有关。我们假设，在疾病诱导后持续存在的重度应激对 CIA 的易感性表现出有益的影响，并提出与重度应激相关的特定内分泌和免疫特征是疾病结果的一个重要因素——这一因素可能解释了 CIA 之前应激研究中许多相互矛盾的数据。
