Departamento de Medicina Tropical, Programa de Pós-Graduação em Medicina Tropical, Universidade Federal de Pernambuco, Recife, PE.
Rev Soc Bras Med Trop. 2012 Dec;45(6):707-12. doi: 10.1590/s0037-86822012000600010.
The emergence of carbapenem resistance mechanisms in Pseudomonas aeruginosa has been outstanding due to the wide spectrum of antimicrobial degradation of these bacteria, reducing of therapeutic options.
Sixty-one clinical strains of P. aeruginosa isolated from five public hospitals in Recife, Pernambuco, Brazil, were examined between 2006 and 2010, aiming of evaluating the profiles of virulence, resistance to antimicrobials, presence of metallo-β-lactamase (MBL) genes, and clonal relationship among isolates.
A high percentage of virulence factors (34.4% mucoid colonies; 70.5% pyocyanin; 93.4% gelatinase positives; and 72.1% hemolysin positive) and a high percentage of antimicrobial resistance rates (4.9% pan-resistant and 54.1% multi-drug resistant isolates) were observed. Among the 29 isolates resistant to imipenem and/or ceftazidime, 44.8% (13/29) were MBL producers by phenotypic evaluation, and of these, 46.2% (6/13) were positive for the blaSPM-1 gene. The blaIMP and blaVIM genes were not detected. The molecular typing revealed 21 molecular profiles of which seven were detected in distinct hospitals and periods. Among the six positive blaSPM-1 isolates, three presented the same clonal profile and were from the same hospital, whereas the other three presented different clonal profiles.
These results revealed that P. aeruginosa is able to accumulate different resistance and virulence factors, making the treatment of infections difficult. The identification of blaSPM-1 genes and the dissemination of clones in different hospitals, indicate the need for stricter application of infection control measures in hospitals in Recife, Brazil, aiming at reducing costs and damages caused by P. aeruginosa infections.
铜绿假单胞菌对抗菌药物的广谱降解作用导致其产生碳青霉烯类耐药机制，这降低了治疗选择。
2006 年至 2010 年间，我们检测了来自巴西累西腓市五家公立医院的 61 株临床分离的铜绿假单胞菌，旨在评估其毒力、对抗菌药物的耐药性、金属β-内酰胺酶（MBL）基因的存在情况，以及分离株之间的克隆关系。
观察到高比例的毒力因子（34.4%粘液性菌落；70.5%绿脓菌素；93.4%明胶酶阳性；72.1%溶血素阳性）和高比例的抗菌药物耐药率（4.9%泛耐药和 54.1%多药耐药分离株）。在 29 株对亚胺培南和/或头孢他啶耐药的分离株中，通过表型评估，有 44.8%（13/29）为 MBL 产生者，其中 46.2%（6/13）blaSPM-1 基因阳性。未检测到 blaIMP 和 blaVIM 基因。分子分型显示 21 种分子谱型，其中 7 种在不同的医院和时期中检测到。在 6 株blaSPM-1 阳性分离株中，有 3 株具有相同的克隆谱型，且来自同一医院，而其他 3 株则具有不同的克隆谱型。
这些结果表明，铜绿假单胞菌能够积累不同的耐药和毒力因子，使感染的治疗变得困难。blaSPM-1 基因的鉴定和克隆在不同医院的传播表明，巴西累西腓市的医院需要更严格地应用感染控制措施，以降低铜绿假单胞菌感染造成的成本和损害。
