Malmgren A, Uvelius B, Andersson K E, Andersson P O
Department of Clinical Pharmacology, University of Lund, Sweden.
J Urol. 1990 May;143(5):1026-31. doi: 10.1016/s0022-5347(17)40176-5.
Rats were subjected to infravesical outflow obstruction for six weeks. The bladder function was followed by cytometrical and in vitro investigations and by recordings of micturition pattern before and after removal of the obstruction. Cytometrical investigations showed that outflow obstruction for six weeks induced a bladder instability. Further, in the presence of obstruction the micturition pressure was large as was the bladder capacity and the rats had residual urine. After removal of the obstruction the bladder function rapidly normalized. The bladder instability disappeared within one week, bladder capacity decreased as did the micturition pressure. Moreover, only a minor amount of residual urine was present post-obstruction. In vitro investigation showed that the response to carbachol and to electrical stimulation was similar in normal and obstructed bladders. However, after removal of the obstruction a supersensitivity to carbachol as well as to electrical stimulation had developed. Obstructed bladders showed a markedly decreased response to substance P. The sensitivity to substance P was rapidly enhanced post-obstruction and after four days the response was restored to the control level. The present study shows that the bladder function in rats with infravesical outflow obstruction rapidly normalized after removal of the obstruction. The disappearance of the bladder instability despite the developed supersensitivity to muscarinic receptor stimulation supports the opinion that the bladder instability is not of muscarinic origin.
将大鼠进行膀胱颈以下流出道梗阻六周。通过膀胱容量测定和体外研究以及记录梗阻解除前后的排尿模式来跟踪膀胱功能。膀胱容量测定研究表明，六周的流出道梗阻会导致膀胱不稳定。此外，在存在梗阻的情况下，排尿压力和膀胱容量都很大，并且大鼠有残余尿。梗阻解除后，膀胱功能迅速恢复正常。膀胱不稳定在一周内消失，膀胱容量和排尿压力均下降。此外，梗阻后仅存在少量残余尿。体外研究表明，正常膀胱和梗阻膀胱对卡巴胆碱和电刺激的反应相似。然而，梗阻解除后，对卡巴胆碱和电刺激出现了超敏反应。梗阻膀胱对P物质的反应明显降低。梗阻后对P物质的敏感性迅速增强，四天后反应恢复到对照水平。本研究表明，膀胱颈以下流出道梗阻的大鼠在梗阻解除后膀胱功能迅速恢复正常。尽管对毒蕈碱受体刺激出现了超敏反应，但膀胱不稳定的消失支持了膀胱不稳定并非源于毒蕈碱的观点。
