Research Group on Evidence, Lifestyles and Health, Research Institute on Health Sciences, IUNICS, University of the Balearic Islands, Palma de Mallorca, Spain.
Med Sci Sports Exerc. 2013 Jul;45(7):1269-76. doi: 10.1249/MSS.0b013e3182857c8a.
The objective of this study is as follows: 1) to determine the effects of caffeine supplementation on the inflammatory response (IL-6 and IL-10 levels and leukocyte numbers) induced by a 15-km run competition and 2) to examine the effect of caffeine supplementation on the energetic metabolites as well as on the exercise-induced oxidative stress.
A double-blinded study of supplementation with caffeine was performed. Athletes participating in the study (n = 33) completed a 15-km run competition. Before competition, athletes took 6 mg · kg(-1) body weight of caffeine (caffeine group, n = 17) or a placebo (placebo group, n = 16). Blood samples were taken before and after competition (immediately and after 2-h recovery). Leukocyte numbers were determined in blood. Concentrations of oxidative stress markers, antioxidants, interleukins (IL-6 and IL-10), caffeine, adrenaline, and energetic metabolites were measured in plasma or serum.
Caffeine supplementation induced higher increases in circulating total leukocytes and neutrophils, with significant differences between groups after recovery. Adrenaline, glucose, and lactate levels increased after exercise, with higher increases in the caffeine group. Exercise induced significant increases in IL-6 and IL-10 plasma levels, with higher increases in the caffeine group. Caffeine supplementation induced higher increases in oxidative stress markers after the competition.
Caffeine supplementation induced higher levels of IL-6 and IL-10 in response to exercise, enhancing the anti-inflammatory response. The caffeine-induced increase in adrenaline could be responsible for the higher increase in IL-6 levels, as well as for the increased lactate levels. Furthermore, caffeine seems to enhance oxidative stress induced by exercise.
本研究的目的如下：1）确定咖啡因补充对 15 公里跑步比赛引起的炎症反应（IL-6 和 IL-10 水平和白细胞数量）的影响；2）研究咖啡因补充对能量代谢物以及运动引起的氧化应激的影响。
进行了一项补充咖啡因的双盲研究。参加研究的运动员（n = 33）完成了 15 公里跑步比赛。在比赛前，运动员服用 6 mg·kg(-1)体重的咖啡因（咖啡因组，n = 17）或安慰剂（安慰剂组，n = 16）。在比赛前后（立即和 2 小时恢复后）采集血液样本。测定血液中的白细胞数量。在血浆或血清中测量氧化应激标志物、抗氧化剂、白细胞介素（IL-6 和 IL-10）、咖啡因、肾上腺素和能量代谢物的浓度。
咖啡因补充诱导循环总白细胞和中性粒细胞的增加更高，恢复后组间有显著差异。运动后肾上腺素、葡萄糖和乳酸水平升高，咖啡因组升高更明显。运动诱导 IL-6 和 IL-10 血浆水平显著升高，咖啡因组升高更明显。咖啡因补充在比赛后诱导更高的氧化应激标志物水平。
咖啡因补充诱导运动后更高水平的 IL-6 和 IL-10，增强抗炎反应。咖啡因诱导的肾上腺素增加可能是 IL-6 水平升高以及乳酸水平升高的原因。此外，咖啡因似乎增强了运动引起的氧化应激。
