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用于第二代木质素分析的基质辅助激光解吸/电离质谱法的评估

Evaluation of matrix-assisted laser desorption/ionization mass spectrometry for second-generation lignin analysis.

作者信息

Richel Aurore, Vanderghem Caroline, Simon Mathilde, Wathelet Bernard, Paquot Michel

机构信息

Unit of Biological and Industrial Chemistry, Gembloux Agro-Bio Tech, University of Liege, Gembloux, Belgium.

出版信息

Anal Chem Insights. 2012;7:79-89. doi: 10.4137/ACI.S10799. Epub 2012 Dec 13.

DOI:10.4137/ACI.S10799
PMID:23300342
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3528113/
Abstract

Matrix-Assisted Laser Desorption/Ionization time-of-flight (MALDI-TOF) mass spectrometry is evaluated as an elucidation tool for structural features and molecular weights estimation of some extracted herbaceous lignins. Optimization of analysis conditions, using a typical organic matrix, namely α-cyano-4-hydroxycinnamic acid (CHCA), in combination with α-cyclodextrin, allows efficient ionization of poorly soluble lignin materials and suppression of matrix-related ions background. Analysis of low-mass fragments ions (m/z 100-600) in the positive ion mode offers a "fingerprint" of starting lignins that could be a fine strategy to qualitatively identify principal inter-unit linkages between phenylpropanoid units. The molecular weights of lignins are estimated using size exclusion chromatography and compared to MALDI-TOF-MS profiles. Miscanthus (Miscanthus x giganteus) and Switchgrass (Panicum Virgatum L.) lignins, recovered after a formic acid/acetic acid/water process or aqueous ammonia soaking, are selected as benchmarks for this study.

摘要

基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱法被评估为一种用于阐明某些提取的草本木质素结构特征和估计分子量的工具。使用典型的有机基质，即α-氰基-4-羟基肉桂酸（CHCA），结合α-环糊精，对分析条件进行优化，可实现难溶性木质素材料的有效电离，并抑制与基质相关的离子背景。在正离子模式下分析低质量碎片离子（m/z 100-600）可提供起始木质素的“指纹”，这可能是定性鉴定苯丙烷单元之间主要单元间连接的良好策略。使用尺寸排阻色谱法估计木质素的分子量，并与MALDI-TOF-MS谱图进行比较。在甲酸/乙酸/水工艺或氨水浸泡后回收的芒草（Miscanthus x giganteus）和柳枝稷（Panicum Virgatum L.）木质素被选为本研究的基准。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/47399d5ea4d2/aci-7-2012-079f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/33e186c41f94/aci-7-2012-079f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/aba791ccb7b7/aci-7-2012-079f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/8736229e9b3a/aci-7-2012-079f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/f372144c5e01/aci-7-2012-079f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/323360a4d179/aci-7-2012-079f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/47399d5ea4d2/aci-7-2012-079f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/33e186c41f94/aci-7-2012-079f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/aba791ccb7b7/aci-7-2012-079f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/8736229e9b3a/aci-7-2012-079f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/f372144c5e01/aci-7-2012-079f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/323360a4d179/aci-7-2012-079f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19d9/3528113/47399d5ea4d2/aci-7-2012-079f6.jpg

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