Han Y M, Castanon F, Parsons C M, Baker D H
Department of Animal Sciences, University of Illinois, Urbana-Champaign 61801.
Poult Sci. 1990 Feb;69(2):281-7. doi: 10.3382/ps.0690281.
Intestinal absorption of crystalline DL-methionine (DL-MET) and DL-methionine hydroxy analog calcium (DL-HMA) were determined in a true-digestibility-balance assay using cecectomized (CEC) and sham-operated conventional (CONV) cockerels. The treatments consisted of fasted birds and birds crop-intubated (CI) with 30 g of a corn-soybean meal basal diet (16% CP) supplemented with 0, .2, or .4% of DL-MET or equimolar levels of DL-HMA. There was no detectable free D-MET or L-MET or HMA in the excreta of fasted birds or of those fed the unsupplemented basal diet. The intestinal absorption of DL-HMA was 95.9 +/- .8% (means +/- SE) for CEC and 98.8 +/- .8% for the CONV cockerels. The absorption of DL-MET was approximately 99.7 +/- .2% for the CEC and the CONV cockerels. In a second experiment procedures were developed for a bioavailability assessment by comparing the growth responses to CI and intraperitoneal-injected (IP) DL-MET or DL-HMA in chicks fed a crystalline-amino-acid diet deficient in methionine. Graded increments of pH-adjusted DL-MET or DL-HMA (in water solutions) were administered twice daily in a 7-day growth assay. Slope-ratio analysis indicated that bioavailability (+/- SE) of CI DL-HMA was 91.3 +/- 11.8% relative to the CI DL-MET on an equimolar basis. The bioavailability of CI DL-HMA was similar to that of IP DL-HMA, indicating that the intestinal absorption of DL-HMA was highly efficient.(ABSTRACT TRUNCATED AT 250 WORDS)
采用切除盲肠（CEC）和假手术的传统（CONV）公鸡，通过真消化率平衡试验测定了结晶型DL-蛋氨酸（DL-MET）和DL-蛋氨酸羟基类似物钙（DL-HMA）的肠道吸收情况。处理组包括禁食的鸡以及嗉囊插管（CI）的鸡，后者饲喂30克添加了0%、0.2%或0.4% DL-MET或等摩尔水平DL-HMA的玉米-豆粕基础日粮（16%粗蛋白）。禁食鸡或饲喂未添加基础日粮鸡的排泄物中未检测到游离的D-MET、L-MET或HMA。CEC公鸡对DL-HMA的肠道吸收为95.9±0.8%（均值±标准误），CONV公鸡为98.8±0.8%。CEC和CONV公鸡对DL-MET的吸收约为99.7±0.2%。在第二个实验中，通过比较蛋氨酸缺乏的结晶氨基酸日粮喂养的雏鸡对CI和腹腔注射（IP）DL-MET或DL-HMA的生长反应，制定了生物利用度评估程序。在为期7天的生长试验中，每天两次给予pH值调节后的DL-MET或DL-HMA（水溶液）梯度增量。斜率比分析表明，在等摩尔基础上，CI DL-HMA相对于CI DL-MET的生物利用度（±标准误）为91.3±11.8%。CI DL-HMA的生物利用度与IP DL-HMA相似，表明DL-HMA的肠道吸收效率很高。（摘要截断于250字）
