Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, 350108 Fuzhou, PR China.
Analyst. 2013 Feb 21;138(4):981-90. doi: 10.1039/c2an36500f.
Recent research has looked to develop innovative, powerful and novel biofunctionalized nanoparticles, controlling and tailoring their properties in a very predictable manner to meet the needs of clinic immunoassays in the biomedical field. This minireview briefly summarizes recent advances covering the last 3 years, exploiting nanoparticle-based electrochemical, optical, mass-sensitive, colorimetric and immunodipstick assays. The enormous signal enhancement associated with the use of nanoparticles and formation of nanoparticle-antibody-antigen assemblies provide the basis for sensitive detection of disease-related proteins or biomarkers. Rather than being exhaustive, this minireview focuses on selected examples to illustrate novel concepts and promising applications. Finally, a small amount of speculation of possible future developments in nanoparticle-based immunoassays is provided.
最近的研究致力于开发创新、强大和新颖的生物功能化纳米粒子，以非常可预测的方式控制和调整它们的性质，以满足生物医学领域临床免疫分析的需求。本篇综述简要总结了过去 3 年中利用基于纳米粒子的电化学、光学、质量敏感、比色和免疫层析分析的最新进展。使用纳米粒子和形成纳米粒子-抗体-抗原组装所带来的巨大信号增强为疾病相关蛋白或生物标志物的敏感检测提供了基础。本篇综述并非详尽无遗，而是侧重于选择的实例来说明新的概念和有前途的应用。最后，对基于纳米粒子的免疫分析可能的未来发展进行了少量推测。
