Department of Chemistry, Institute of Sciences, Gazi University , Ankara , Turkey.
Artif Cells Nanomed Biotechnol. 2013 Oct;41(5):327-31. doi: 10.3109/21691401.2012.744993. Epub 2013 Jan 10.
In this study, a new amperometric biosensor for the determination of hypoxanthine was developed. To this aim, polypyrrole-polyvinyl sulphonate films were prepared on the platinum electrode by the electropolymerization of pyrrole in the presence of polyvinyl sulphonate. Xanthine oxidase and uricase enzymes were immobilized in polypyrrole-polyvinyl sulphonate via the entrapment method. Optimum conditions of enzyme electrode were determined. Hypoxanthine detection is based on the oxidation of hydrogen peroxide at +400 mV produced by the enzymatic reaction on the enzyme electrode surface. The linear working range of biosensor for hypoxanthine was determined. The effects of pH and temperature on the response of the hypoxanthine biosensor were investigated. Optimum pH and temperature were measured as 8 and 30°C, respectively. Operational and storage stability of the biosensor were determined. After 20 assays, the biosensor sustained 74.5% of its initial performance. After 33 days, the biosensor lost 36% of its initial performance. The performance of the biosensor was tested in real samples.
在这项研究中，开发了一种用于测定次黄嘌呤的新电流型生物传感器。为此，通过在存在聚磺酸钠的情况下聚合吡咯，在铂电极上制备了聚吡咯-聚磺酸钠膜。黄嘌呤氧化酶和尿酸酶通过包埋法固定在聚吡咯-聚磺酸钠中。确定了酶电极的最佳条件。次黄嘌呤的检测是基于在酶电极表面的酶反应产生的过氧化氢在+400 mV 处的氧化。确定了生物传感器对次黄嘌呤的线性工作范围。研究了 pH 值和温度对次黄嘌呤生物传感器响应的影响。最佳 pH 值和温度分别为 8 和 30°C。确定了生物传感器的操作和储存稳定性。经过 20 次测定后，生物传感器保持了初始性能的 74.5%。33 天后，生物传感器失去了初始性能的 36%。在实际样品中测试了生物传感器的性能。
