Robinson Mark James, Tuke Philip William, Erlwein Otto, Tettmar Kate I, Kaye Steve, Naresh Kikkeri N, Patel Anup, Walker Marjorie M, Kimura Takahiro, Gopalakrishnan Ganesh, Tedder Richard S, McClure Myra O
Section of Infectious Diseases, Jefferiss Research Trust Laboratories, Imperial College London, St Mary's Campus, London W2 1PG, UK.
Adv Virol. 2011;2011:782353. doi: 10.1155/2011/782353. Epub 2011 Jun 9.
Xenotropic murine leukaemia virus-related virus (XMRV) is a recently described retrovirus which has been claimed to infect humans and cause associated pathology. Initially identified in the US in patients with prostate cancer and subsequently in patients with chronic fatigue syndrome, doubt now exists that XMRV is a human pathogen. We studied the prevalence of genetic sequences of XMRV and related MuLV sequences in human prostate cancer, from B cell lymphoma patients and from UK blood donors. Nucleic acid was extracted from fresh prostate tissue biopsies, formalin-fixed paraffin-embedded (FFPE) prostate tissue and FFPE B-cell lymphoma. The presence of XMRV-specific LTR or MuLV generic gag-like sequences was investigated by nested PCR. To control for mouse DNA contamination, a PCR that detected intracisternal A-type particle (IAP) sequences was included. In addition, DNA and RNA were extracted from whole blood taken from UK blood donors and screened for XMRV sequences by real-time PCR. XMRV or MuLV-like sequences were not amplified from tissue samples. Occasionally MuLV gag and XMRV-LTR sequences were amplified from Indian prostate cancer samples, but were always detected in conjunction with contaminating murine genomic DNA. We found no evidence of XMRV or MuLV infection in the UK blood donors.
嗜异性小鼠白血病病毒相关病毒（XMRV）是一种最近被描述的逆转录病毒，据称可感染人类并引发相关病理状况。最初在美国的前列腺癌患者中被发现，随后在慢性疲劳综合征患者中也有发现，现在人们对XMRV是否为人类病原体存在疑问。我们研究了XMRV的基因序列以及相关鼠白血病病毒（MuLV）序列在人类前列腺癌、B细胞淋巴瘤患者以及英国献血者中的流行情况。从新鲜前列腺组织活检样本、福尔马林固定石蜡包埋（FFPE）前列腺组织以及FFPE B细胞淋巴瘤中提取核酸。通过巢式PCR检测XMRV特异性长末端重复序列（LTR）或MuLV通用gag样序列的存在。为了控制小鼠DNA污染，加入了一个检测胞内A型颗粒（IAP）序列的PCR。此外，从英国献血者采集的全血中提取DNA和RNA，并通过实时PCR筛选XMRV序列。未从组织样本中扩增出XMRV或MuLV样序列。偶尔从印度前列腺癌样本中扩增出MuLV gag和XMRV-LTR序列，但总是与污染的小鼠基因组DNA一起被检测到。我们在英国献血者中未发现XMRV或MuLV感染的证据。
