未能在慢性疲劳综合征患者的血液中确认 XMRV/MLVs：一项多实验室研究。
Failure to confirm XMRV/MLVs in the blood of patients with chronic fatigue syndrome: a multi-laboratory study.
机构信息
Blood Systems Research Institute and University of California, San Francisco, San Francisco, CA 94118, USA.
出版信息
Science. 2011 Nov 11;334(6057):814-7. doi: 10.1126/science.1213841. Epub 2011 Sep 22.
Abstract
Murine leukemia viruses (MLVs), including xenotropic-MLV-related virus (XMRV), have been controversially linked to chronic fatigue syndrome (CFS). To explore this issue in greater depth, we compiled coded replicate samples of blood from 15 subjects previously reported to be XMRV/MLV-positive (14 with CFS) and from 15 healthy donors previously determined to be negative for the viruses. These samples were distributed in a blinded fashion to nine laboratories, which performed assays designed to detect XMRV/MLV nucleic acid, virus replication, and antibody. Only two laboratories reported evidence of XMRV/MLVs; however, replicate sample results showed disagreement, and reactivity was similar among CFS subjects and negative controls. These results indicate that current assays do not reproducibly detect XMRV/MLV in blood samples and that blood donor screening is not warranted.
摘要
鼠白血病病毒（MLV），包括嗜性 MLV 相关病毒（XMRV），与慢性疲劳综合征（CFS）有争议的关联。为了更深入地探讨这个问题，我们编译了编码的 15 名先前报道为 XMRV/MLV 阳性（14 名患有 CFS）和 15 名先前确定为病毒阴性的健康供体的血液重复样本。这些样本以盲法分发给九家实验室，这些实验室进行了旨在检测 XMRV/MLV 核酸、病毒复制和抗体的检测。只有两家实验室报告了 XMRV/MLV 的证据；然而，重复样本的结果显示存在分歧，并且 CFS 患者和阴性对照之间的反应性相似。这些结果表明，目前的检测方法不能在血液样本中重复检测到 XMRV/MLV，并且不需要对献血者进行筛查。
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本文引用的文献
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Phylogenetic analysis of murine leukemia virus sequences from longitudinally sampled chronic fatigue syndrome patients suggests PCR contamination rather than viral evolution.从纵向采样的慢性疲劳综合征患者中分离出的鼠白血病病毒序列的系统进化分析提示聚合酶链反应污染而非病毒进化。
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Recombinant origin of the retrovirus XMRV.逆转录病毒 XMRV 的重组起源。
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No association of xenotropic murine leukemia virus-related viruses with prostate cancer.未发现嗜异性鼠白血病病毒相关病毒与前列腺癌有关。
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