Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA.
Phytochemistry. 2013 Jul;91:208-19. doi: 10.1016/j.phytochem.2012.11.012. Epub 2013 Jan 7.
A UPLC-MS method was developed for quantifying huperzine A (HupA), an anti-Alzheimer's disease (AD) drug candidate from the traditional Chinese medicine Qian Ceng Ta (Huperzia serrata), in samples of 11 Huperzia genus plants. The highest content of HupA was found in Huperzia pinifolia. The accumulation of various Lycopodium alkaloids was monitored in these tissues using high resolution Q-IMS-TOFMS analysis. Tissue culture of various Huperzia species has been achieved and production of HupA has been confirmed in the callus of H. pinifolia. Furthermore, it was established that the major alkaloid produced by the naturally grown plant and the callus of H. pinifolia changed dramatically from HupA to nankakurine B.
建立了 UPLC-MS 方法，用于对来自传统中药千层塔（蛇足石杉）的抗阿尔茨海默病（AD）候选药物石杉碱甲（HupA）进行定量分析，该方法可用于 11 种蛇足石杉属植物样本。在 Huperzia pinifolia 中发现 HupA 的含量最高。使用高分辨 Q-IMS-TOFMS 分析监测了这些组织中各种石松生物碱的积累情况。已经实现了各种蛇足石杉的组织培养，并在 Huperzia pinifolia 的愈伤组织中确认了 HupA 的产生。此外，已经证实，天然生长的植物和 Huperzia pinifolia 愈伤组织中产生的主要生物碱从 HupA 剧烈转变为石杉碱甲 B。
