Department of Clinical Microbiology and Immunology, College of Medical Laboratory Science, Third Military Medical University, Chongqing 400038, PR China.
Vaccine. 2013 Feb 6;31(8):1204-9. doi: 10.1016/j.vaccine.2012.12.058. Epub 2013 Jan 7.
An epitope-based vaccine is a promising option for treating Helicobacter pylori (H. pylori) infection. Epitope mapping is the first step in designing an epitope-based vaccine. A pivotal role of CD4(+) T cells in protection against H. pylori has been accepted, but few Th epitopes have been identified. In this study, two novel UreB CD4(+) T cell epitopes were identified using PBMCs obtained from two H. pylori infected subjects. We determined the restriction molecules by antibody blocking and used various Epstein-Barr virus-transformed B lymphocyte cell lines (BLCLs) with different HLA alleles as APCs to present peptides to CD4(+) T cells. These epitopes were DRB11404-restricted UreB(373-385) and DRB10803-restricted UreB(438-452). The T cells specific to these epitopes not only recognized autologous DCs loaded with recombinant UreB but also those pulsed with H. pylori whole cell lysates, suggesting that these epitope peptides are naturally processed. These epitopes have important value for designing an effective H. pylori vaccine.
基于表位的疫苗是治疗幽门螺杆菌（H. pylori）感染的一种有前途的选择。表位作图是设计基于表位的疫苗的第一步。CD4(+) T 细胞在预防 H. pylori 中的关键作用已被接受，但很少有 Th 表位被鉴定出来。在这项研究中，使用来自两名 H. pylori 感染受试者的 PBMC 鉴定了两种新型 UreB CD4(+) T 细胞表位。我们通过抗体阻断确定了限制分子，并使用具有不同 HLA 等位基因的各种 Epstein-Barr 病毒转化的 B 淋巴细胞系（BLCL）作为 APC 来将肽呈递给 CD4(+) T 细胞。这些表位是 DRB11404 限制性 UreB(373-385)和 DRB10803 限制性 UreB(438-452)。针对这些表位的 T 细胞不仅识别负载重组 UreB 的自体 DC，还识别用 H. pylori 全细胞裂解物脉冲的 DC，表明这些表位肽是自然加工的。这些表位对于设计有效的 H. pylori 疫苗具有重要价值。
