Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, L69 3BX, UK.
Cell Mol Life Sci. 2013 Sep;70(18):3423-33. doi: 10.1007/s00018-012-1250-8. Epub 2013 Jan 11.
Endocytosis and mitosis are fundamental processes in a cell's life. Nearly 50 years of research suggest that these processes are linked and that endocytosis is shut down as cells undergo the early stages of mitosis. Precisely how this occurs at the molecular level is an open question. In this review, we summarize the early work characterizing the inhibition of clathrin-mediated endocytosis and discuss recent challenges to this established concept. We also set out four proposed mechanisms for the inhibition: mitotic phosphorylation of endocytic proteins, altered membrane tension, moonlighting of endocytic proteins, and a mitotic spindle-dependent mechanism. Finally, we speculate on the functional consequences of endocytic shutdown during mitosis and where an understanding of the mechanism of inhibition will lead us in the future.
内吞作用和有丝分裂是细胞生命中的基本过程。近 50 年的研究表明，这些过程是相关的，并且在细胞经历有丝分裂的早期阶段时，内吞作用被关闭。在分子水平上，这种情况是如何发生的，这是一个悬而未决的问题。在这篇综述中，我们总结了早期表征网格蛋白介导的内吞作用抑制的工作，并讨论了这一既定概念的最新挑战。我们还提出了四种内吞作用抑制的机制：内吞作用蛋白的有丝分裂磷酸化、膜张力改变、内吞作用蛋白的兼职、以及有丝分裂纺锤体依赖性机制。最后，我们推测了有丝分裂过程中内吞作用关闭的功能后果，以及对抑制机制的理解将在未来引领我们走向何方。
