Department of Cellular and Molecular Physiology, Institute of Translational Medicine, University of Liverpool, Liverpool, UK.
J Cell Sci. 2012 Jan 1;125(Pt 1):19-28. doi: 10.1242/jcs.094607.
Clathrin, a protein best known for its role in membrane trafficking, has been recognised for many years as localising to the spindle apparatus during mitosis, but its function at the spindle remained unclear. Recent work has better defined the role of clathrin in the function of the mitotic spindle and proposed that clathrin crosslinks the microtubules (MTs) comprising the kinetochore fibres (K-fibres) in the mitotic spindle. This mitotic function is unrelated to the role of clathrin in membrane trafficking and occurs in partnership with two other spindle proteins: transforming acidic coiled-coil protein 3 (TACC3) and colonic hepatic tumour overexpressed gene (ch-TOG; also known as cytoskeleton-associated protein 5, CKAP5). This review summarises the role of clathrin in mitotic spindle organisation with an emphasis on the recent discovery of the TACC3-ch-TOG-clathrin complex.
网格蛋白，一种以其在膜运输中的作用而闻名的蛋白质，多年来一直被认为在有丝分裂期间定位于纺锤体装置，但它在纺锤体中的功能仍不清楚。最近的工作更好地定义了网格蛋白在有丝分裂纺锤体功能中的作用，并提出网格蛋白交联构成有丝分裂纺锤体的动粒纤维（K-纤维）的微管（MTs）。这种有丝分裂功能与网格蛋白在膜运输中的作用无关，它与另外两种纺锤体蛋白：转化酸性卷曲螺旋蛋白 3（TACC3）和结肠肝肿瘤过表达基因（ch-TOG；也称为细胞骨架相关蛋白 5，CKAP5）合作发生。本文综述了网格蛋白在有丝分裂纺锤体组织中的作用，重点介绍了 TACC3-ch-TOG-网格蛋白复合物的最新发现。
