Department of Morphology, Piracicaba Dental School (FOP), University of Campinas (UNICAMP), Av. Limeira, 901, 13414-903, Piracicaba, SP, Brazil,
Lasers Med Sci. 2013 Nov;28(6):1467-74. doi: 10.1007/s10103-012-1251-8. Epub 2013 Jan 10.
The aim of this study was to histologically and biochemically analyze the effects of light-emitting diode therapy (LEDT) associated with resistance training to prevent sarcopenia in ovariectomized rats. Forty female Wistar rats (12 months old, 295-330 g) were bilaterally ovariectomized and divided into four groups (n = 10 per group): control-sedentary (C), resistance training (T), LEDT-sedentary (L), and LEDT plus resistance training (LT). Trained rats performed a 12-week water-jumping program (3 days per week) carrying a load equivalent to 50-80% of their body mass strapped to their back. Depending on the group protocol, the LED device (850 nm, 100 mW, 120 J/cm(2), spot size 0.5 cm(2)) was used either as the only method or after the resistance training had been performed. The device was used in the single point contact mode (for 10 min). The irradiated region was the center of the greater trochanter of the right femur and the middle third of the rectus femoris muscle was subsequently analyzed histomorphometrically. Significant increases (p < 0.05) were noted for the muscle volume of the T (68.1 ± 19.7%), the L (74.1 ± 5.1%), and the LT (68.2 ± 11.5%) groups compared to the C group (60.4 ± 5.5%). There were also significant increases in the concentrations of IGF-1, IL-1, and TNF-α in the muscles of the treated groups (p < 0.05). Animals in the LT group showed a significant increase in IL-6 compared to T, L, and C groups (p < 0.05). These findings suggest that resistance training and LEDT can prevent sarcopenia in ovariectomized rats.
本研究旨在从组织学和生物化学方面分析发光二极管疗法（LEDT）联合抗阻训练对去卵巢大鼠预防肌肉减少症的效果。将 40 只 12 月龄、体质量为 295-330 g 的雌性 Wistar 大鼠双侧去卵巢，分为 4 组（每组 10 只）：对照组（C）、抗阻训练组（T）、LEDT 联合对照组（L）和 LEDT 联合抗阻训练组（LT）。训练大鼠每周 3 天进行水跳训练（负荷相当于其体质量的 50-80%）。根据组间方案，将 LED 装置（850nm，100mW，120J/cm(2)，光斑尺寸 0.5cm(2)）用于单一接触点（10min）或在完成抗阻训练后用于大鼠。照射区域为右股骨大转子中心和股直肌中部三分之一，随后进行组织形态计量学分析。与 C 组（60.4±5.5%）相比，T 组（68.1±19.7%）、L 组（74.1±5.1%）和 LT 组（68.2±11.5%）的肌肉体积均显著增加（p<0.05）。此外，治疗组肌肉中 IGF-1、IL-1 和 TNF-α 的浓度也显著增加（p<0.05）。与 T、L 和 C 组相比，LT 组的 IL-6 显著增加（p<0.05）。这些发现表明，抗阻训练和 LEDT 可预防去卵巢大鼠的肌肉减少症。
