Saul D, Kling J H, Kosinsky R L, Hoffmann D B, Komrakova M, Wicke M, Menger B, Sehmisch S
Department of Trauma, Orthopedics and Reconstructive Surgery, Georg-August-University of Göttingen, Göttingen, Germany.
Department of General, Visceral and Pediatric Surgery, University Medical Center Göttingen, 37075 Göttingen, Germany.
J Nutr Metab. 2016;2016:3703216. doi: 10.1155/2016/3703216. Epub 2016 Dec 5.
Sarcopenia, a loss of muscle mass accompanying osteoporosis, leads to falls and fall-related injuries. Baicalein, as a phytochemical agent, has an antioxidative and anti-inflammatory effect in muscle. In this study, sixty-one female Sprague Dawley rats were divided into five groups: four groups were ovariectomized (OVX) and one control group was nonovariectomized (NON-OVX). Eight weeks after ovariectomy, three disparate concentrations (1 mg/kg body weight (BW), 10 mg/kg BW, and 100 mg/kg BW) of baicalein were applied subcutaneously daily in three OVX groups. Mm. soleus, gastrocnemius, and longissimus were extracted; their diameter, area, relation to body, and muscle weights as well as number of capillaries per fibre were recorded. In Mm. soleus and gastrocnemius, the baicalein effect (increasing number of capillaries per fibre) was proportional to the dose applied. The fibre diameters and area under baicalein treatment were significantly greater compared to OVX and NON-OVX groups. In M. longissimus, we observed a shift to type IIa fibres. Serum creatine kinase levels were significantly lower in highest baicalein concentration group. We conclude that baicalein can stimulate angiogenesis, though not fibre type-specific, in skeletal muscle and reduce the estrogen-related loss of fibre diameter and area in the skeletal muscle in rats. Therefore, a protective effect of baicalein on muscle cells can be assumed.
肌肉减少症是一种伴随骨质疏松症出现的肌肉量流失现象，会导致跌倒及与跌倒相关的损伤。黄芩苷作为一种植物化学制剂，在肌肉中具有抗氧化和抗炎作用。在本研究中，61只雌性斯普拉格-道利大鼠被分为五组：四组进行卵巢切除（OVX），一组作为对照组不进行卵巢切除（NON-OVX）。卵巢切除术后8周，对三个OVX组每天皮下注射三种不同浓度（1毫克/千克体重（BW）、10毫克/千克BW和100毫克/千克BW）的黄芩苷。提取比目鱼肌、腓肠肌和背最长肌；记录它们的直径、面积、与身体的关系、肌肉重量以及每根纤维的毛细血管数量。在比目鱼肌和腓肠肌中，黄芩苷的作用（增加每根纤维的毛细血管数量）与给药剂量成正比。与OVX组和NON-OVX组相比，黄芩苷处理组的纤维直径和面积显著更大。在背最长肌中，我们观察到向IIa型纤维的转变。最高黄芩苷浓度组的血清肌酸激酶水平显著降低。我们得出结论，黄芩苷可以刺激大鼠骨骼肌中的血管生成，尽管不是纤维类型特异性的，并且可以减少与雌激素相关的骨骼肌纤维直径和面积的损失。因此，可以推测黄芩苷对肌肉细胞具有保护作用。
