Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, P,O, box 30001, Groningen, 9700 RB, the Netherlands.
Microb Cell Fact. 2013 Jan 14;12:3. doi: 10.1186/1475-2859-12-3.
The biotechnology industry has become a key element in modern societies. Within this industry, the production of recombinant enzymes and biopharmaceutical proteins is of major importance. The global markets for such recombinant proteins are growing rapidly and, accordingly, there is a continuous need for new production platforms that can deliver protein products in greater yields, with higher quality and at lower costs. This calls for the development of next-generation super-secreting cell factories. One of the microbial cell factories that can meet these challenges is the Gram-positive bacterium Bacillus subtilis, an inhabitant of the upper layers of the soil that has the capacity to secrete proteins in the gram per litre range. The engineering of B. subtilis into a next-generation super-secreting cell factory requires combined Systems and Synthetic Biology approaches. In this way, the bacterial protein secretion machinery can be optimized from the single molecule to the network level while, at the same time, taking into account the balanced use of cellular resources. Although highly ambitious, this is an achievable objective due to recent advances in functional genomics and Systems- and Synthetic Biological analyses of B. subtilis cells.
生物技术产业已成为现代社会的重要组成部分。在这个行业中，重组酶和生物制药蛋白的生产具有重要意义。此类重组蛋白的全球市场正在迅速增长，因此，不断需要新的生产平台，以更高的产量、更高的质量和更低的成本提供蛋白产品。这就需要开发下一代超分泌细胞工厂。能够应对这些挑战的微生物细胞工厂之一是革兰氏阳性细菌枯草芽孢杆菌，它是土壤上层的一种栖息者，能够在每升范围内分泌蛋白。将枯草芽孢杆菌工程化为下一代超分泌细胞工厂需要结合系统和合成生物学方法。通过这种方式，可以从单个分子到网络水平优化细菌蛋白分泌机制，同时考虑到细胞资源的平衡利用。尽管这一目标极具挑战性，但由于近年来在枯草芽孢杆菌细胞的功能基因组学和系统及合成生物学分析方面取得了进展，这一目标是可以实现的。
