Department of Microbiology, Faculty of Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
Drug Deliv. 2013;20(1):34-9. doi: 10.3109/10717544.2012.746402.
Even though the therapeutic efficacy of numerous antimicrobial drugs has been well established, inefficient delivery can result in an inadequate therapeutic index. Gold nanoparticles have unique physicochemical properties such as large surface area to mass ratio and functionalizable structure. These properties can be applied to facilitate the administration of antimicrobial drugs, thereby overcoming some of the limitations in traditional antimicrobial therapeutics. In this study, gold nanospheres were used as a drug carrier system for gentamicin delivery to Staphylococcal infected foci. Conjugation of gentamicin with gold nanospheres was performed in HEPES buffer. The attachment of gentamicin to gold nanospheres was confirmed by UV/Vis spectroscopy. The HPLC and atomic absorption spectrometer analyses showed that 347 gentamicin molecules were attached to each gold nanosphere. Minimum inhibitory concentration and minimum bactericidal concentration studies showed the enhanced antibacterial effect of gentamicin-gold nanospheres complex in comparison with free gentamicin. The biodistribution study showed the localization of the complex at the site of Staphylococcal infection foci with high sensitivity in mouse model.
尽管许多抗菌药物的治疗效果已经得到很好的确立，但低效的传递可能导致治疗指数不足。金纳米颗粒具有独特的物理化学性质，如大的表面积与质量比和可功能化的结构。这些特性可应用于促进抗菌药物的给药，从而克服传统抗菌治疗中的一些限制。在这项研究中，金纳米球被用作庆大霉素递送至葡萄球菌感染病灶的药物载体系统。庆大霉素与金纳米球的连接是在 HEPES 缓冲液中进行的。通过紫外/可见光谱证实了庆大霉素与金纳米球的连接。HPLC 和原子吸收光谱分析表明，每个金纳米球上附着了 347 个庆大霉素分子。最低抑菌浓度和最低杀菌浓度研究表明，与游离庆大霉素相比，庆大霉素-金纳米球复合物具有增强的抗菌作用。生物分布研究表明，该复合物在小鼠模型中能够在葡萄球菌感染病灶部位高度敏感地定位。
