Laboratory of Stem Cell and Tissue Engineering, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, PR China.
Cytotherapy. 2013 Mar;15(3):323-9. doi: 10.1016/j.jcyt.2012.11.011. Epub 2013 Jan 9.
Bone marrow-derived mesenchymal stem cells (BM-MSCs) hold great promise for tissue regeneration. With increasing numbers of clinical trials, the safety of BM-MSCs attracts great interest. Previously, we determined that rat BM-MSCs possessed spontaneous calcification without osteogenic induction after continuous culture. However, it is unclear whether BM-MSCs from other species share this characteristic. In this study, spontaneous calcification of BM-MSCs from rat, goat, and human specimens was investigated in vitro. BM-MSCs were cultured in complete medium, and calcification was determined by morphologic observation and alizarin red staining. It was demonstrated that rat BM-MSCs possessed a typically spontaneous calcification, whereas goat and human BM-MSCs under the same system proliferated significantly but did not calcify spontaneously. The significant species variation in spontaneous calcification of BM-MSCs described in this study provides useful information regarding evaluation of numerous BM-MSC-based approaches for bone regeneration and the safety of BM-MSCs.
骨髓间充质干细胞（BM-MSCs）在组织再生方面具有巨大的应用潜力。随着越来越多的临床试验的开展，BM-MSCs 的安全性引起了广泛关注。先前，我们已经确定，大鼠 BM-MSCs 在没有成骨诱导的情况下，经过连续培养会自发钙化。然而，其他物种的 BM-MSCs 是否具有这一特性尚不清楚。在本研究中，我们在体外研究了来自大鼠、山羊和人标本的 BM-MSCs 的自发钙化情况。将 BM-MSCs 培养在完全培养基中，通过形态学观察和茜素红染色来确定钙化情况。结果表明，大鼠 BM-MSCs 具有典型的自发钙化特性，而在相同的培养体系中，山羊和人 BM-MSCs 显著增殖但不会自发钙化。本研究中描述的 BM-MSCs 自发钙化的显著种属差异为评估大量基于 BM-MSCs 的骨再生方法和 BM-MSCs 的安全性提供了有用的信息。
