National Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba 305-8518, Japan.
J Proteomics. 2013 Feb 21;79:231-50. doi: 10.1016/j.jprot.2012.12.023. Epub 2013 Jan 9.
Flooding stress of soybean is a serious problem because it reduces growth; however, flooding-tolerant cultivars have not been identified. To analyze the flooding tolerance mechanism of soybean, the flooding-tolerant mutant was isolated and analyzed using a proteomic technique. Flooding-tolerance tests were repeated five times using gamma-ray irradiated soybeans, whose root growth (M6 stage) was not suppressed even under flooding stress. Two-day-old wild-type and mutant plants were subjected to flooding stress for 2days, and proteins were identified using a gel-based proteomic technique. In wild-type under flooding stress, levels of proteins related to development, protein synthesis/degradation, secondary metabolism, and the cell wall changed; however, these proteins did not markedly differ in the mutant. In contrast, an increased number of fermentation-related proteins were identified in the mutant under flooding stress. The root tips of mutant plants were not affected by flooding stress, even though the wild-type plants had damaged root. Alcohol dehydrogenase activity in the mutant increased at an early stage of flooding stress compared with that of the wild-type. Taken together, these results suggest that activation of the fermentation system in the early stages of flooding may be an important factor for the acquisition of flooding tolerance in soybean.
大豆涝害是一个严重的问题，因为它会抑制生长；然而，目前还没有鉴定出耐涝品种。为了分析大豆的耐涝机制，本研究使用蛋白质组学技术分离和分析了耐涝突变体。使用伽马射线辐照大豆重复进行了五次耐涝性试验，即使在涝胁迫下，其根生长（M6 期）也未受到抑制。将两天大的野生型和突变型植株置于涝胁迫下 2 天，然后使用基于凝胶的蛋白质组学技术鉴定蛋白质。在涝胁迫下，野生型中与发育、蛋白质合成/降解、次生代谢和细胞壁相关的蛋白质的水平发生了变化；然而，在突变体中，这些蛋白质没有明显差异。相比之下，在涝胁迫下，突变体中鉴定到了更多与发酵相关的蛋白质。尽管野生型植物的根受损，但突变体的根尖不受涝胁迫的影响。与野生型相比，突变体在涝胁迫早期的醇脱氢酶活性增加。综上所述，这些结果表明，在涝胁迫早期发酵系统的激活可能是大豆获得耐涝性的一个重要因素。
