Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Viçosa, Minas Gerais 36570-000, Brazil.
Bioresour Technol. 2013 Feb;130:296-305. doi: 10.1016/j.biortech.2012.11.140. Epub 2012 Dec 8.
The plant pathogenic fungus Chrysoporthe cubensis was cultivated under solid state employing different substrates and the highest endoglucanase (33.84Ug(-1)), FPase (2.52Ug(-1)), β-glucosidase (21.55Ug(-1)) and xylanase (362.38Ug(-1)) activities were obtained using wheat bran as carbon source. Cellulases and xylanase produced by C. cubensis showed maximal hydrolysis rate at pH 4.0 and in a temperature range of 50-60°C. All enzymatic activities were highly stable at 40 and 50°C through 48h of pre-incubation. Saccharification of alkaline pretreated sugarcane bagasse by crude enzyme extract from C. cubensis resulted in release of 320.8mg/g and 288.7mg/g of glucose and xylose, respectively. On another hand, a similar assay employing commercial cellulase preparation resulted in release of 250.6mg/g and 62.1mg/g of glucose and xylose, respectively. Cellulolytic extract from C. cubensis showed a great potential to be used in biomass saccharification processes.
利用不同的基质，在固态下培养植物病原真菌长枝木霉，以麦麸作为碳源时，内切葡聚糖酶（33.84Ug(-1)）、纤维二糖酶（2.52Ug(-1)）、β-葡萄糖苷酶（21.55Ug(-1)）和木聚糖酶（362.38Ug(-1)）活性最高。C. cubensis 产生的纤维素酶和木聚糖酶在 pH4.0 和 50-60°C 的温度范围内表现出最大的水解速率。所有酶活性在 40 和 50°C 下经过 48 小时的预孵育后，均具有高度稳定性。用 C. cubensis 的粗酶提取物对碱性预处理的甘蔗渣进行糖化，分别释放出 320.8mg/g 和 288.7mg/g 的葡萄糖和木糖。另一方面，采用商业纤维素酶制剂进行类似的测定，分别释放出 250.6mg/g 和 62.1mg/g 的葡萄糖和木糖。C. cubensis 的纤维素酶提取物在生物质糖化过程中有很大的应用潜力。
