Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Campus Universitário, Viçosa, MG, Brazil.
Bioresour Technol. 2013 Sep;144:587-94. doi: 10.1016/j.biortech.2013.07.015. Epub 2013 Jul 11.
Blending of the enzyme extracts produced by different fungi can result in favorable synergetic enhancement of the enzyme blend with regards to the main cellulase activities, as well as the inclusion of accessory enzymes that may not be as abundant in enzyme extracts produced by predominantly cellulase producing fungi. The Chrysoporthe cubensis:Penicillium pinophilum 50:50 (v/v) blend produced herein presented good synergy, especially for FPase and endoglucanase activities which were 76% and 48% greater than theoretical, respectively. This enzyme blend was applied to sugarcane bagasse previously submitted to a simple alkali pretreatment. Glucan hydrolysis efficiency reached an excess of 60% and xylan conversion exceeded 90%. Increasing the hydrolysis temperature from 45 to 50°C also resulted in a 16-20% increase in conversion of both glucan and xylan fractions. The blended enzyme extract obtained therefore showed great potential for application in the lignocellulose hydrolysis process.
不同真菌的酶提取物混合可以产生有利的协同作用，使酶混合物在主要纤维素酶活性方面得到增强，并且包含可能在主要产生纤维素酶的真菌的酶提取物中不那么丰富的辅助酶。本文中所制备的 Chrysoporthe cubensis:Penicillium pinophilum 50:50（v/v）混合物表现出良好的协同作用，尤其是对 FPase 和内切葡聚糖酶活性的协同作用，分别比理论值高 76%和 48%。该酶混合物应用于先前经过简单碱预处理的甘蔗渣。葡聚糖水解效率达到了超过 60%，木聚糖转化率超过 90%。将水解温度从 45°C 提高到 50°C 也导致了葡聚糖和木聚糖部分的转化率提高了 16-20%。因此，所获得的混合酶提取物在木质纤维素水解过程中有很大的应用潜力。
