Stice S L, Robl J M
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst 01003.
Mol Reprod Dev. 1990 Mar;25(3):272-80. doi: 10.1002/mrd.1080250309.
In this study a fraction was prepared from rabbit sperm that activated rabbit and mouse oocytes following injection into the cytoplasm. The sperm factor activated oocytes exhibited cortical granule exocytosis, pronuclear formation, and cleavage. The sperm factor was soluble in aqueous solution and was not active extracellularly. Unlike most artificial activation methods that are only effective with aged oocytes, the sperm factor activated recently ovulated oocytes. The factor appears to be a protein or associated with a protein but not an acrosomal protein. Fractions from both mouse and bull sperm did not activate rabbit or mouse oocytes. Their inactivity may be owing to the techniques used to recover the fractions or differences between species in sperm morphology and fertilization processes. These observations support the hypothesis that oocyte activation is induced by a factor within sperm that is released into the cytoplasm of the oocyte at the time of sperm-oocyte fusion.
在本研究中，从兔精子中制备了一种组分，将其注入细胞质后可激活兔和小鼠的卵母细胞。被精子因子激活的卵母细胞表现出皮质颗粒胞吐、原核形成和卵裂。精子因子可溶于水溶液，在细胞外无活性。与大多数仅对老化卵母细胞有效的人工激活方法不同，精子因子可激活近期排卵的卵母细胞。该因子似乎是一种蛋白质或与一种蛋白质相关，但不是顶体蛋白。来自小鼠和公牛精子的组分均未激活兔或小鼠的卵母细胞。它们的无活性可能归因于用于回收组分的技术，或物种间精子形态和受精过程的差异。这些观察结果支持这样的假设，即卵母细胞激活是由精子中的一种因子诱导的，该因子在精卵融合时释放到卵母细胞的细胞质中。
