Boyde A, Riggs C M
Dept. of Anatomy and Developmental Biology, University College London, U.K.
Bone. 1990;11(1):35-9. doi: 10.1016/8756-3282(90)90069-b.
We describe and validate a simple method for the study of the proportion of collagen fibers (and apatite crystals) in a bone slice parallel with the plane of section. Viewed between crossed circular polarizers, all bone areas with collagen lying more nearly in the plane of section (i.e., transverse [TS] collagen) appear bright whatever its direction in this plane; longitudinal [LS] collagen appears dark, but not as dark as the background. The degree of brightness increases with section thickness, which must therefore be standardized--we chose 100 microns plane parallel sections. We transferred the circularly polarized light [CPL] image via a CCD TV camera to an image analyzing computer. Color-coded maps of the CPL image were used to compare regions within and between sections. The new analytical procedure makes more detailed studies of the fine-structural orientation in compact bone possible, but does this have any significance? To answer this question, a bone in which the in vivo strain pattern had been clearly documented was chosen for particular study. Transverse mid-diaphyseal sections of the equine radius showed a distribution of CPL bright areas which correlated closely with previously reported strain patterns.
我们描述并验证了一种简单的方法，用于研究与切片平面平行的骨切片中胶原纤维（和磷灰石晶体）的比例。在交叉圆偏振器之间观察时，所有胶原纤维更接近切片平面（即横向[TS]胶原）的骨区域无论其在该平面中的方向如何都会显得明亮；纵向[LS]胶原显得黑暗，但不像背景那么暗。亮度程度随切片厚度增加，因此必须对其进行标准化——我们选择了100微米的平行平面切片。我们通过电荷耦合器件电视摄像机将圆偏振光[CPL]图像传输到图像分析计算机。CPL图像的彩色编码图用于比较切片内和切片间的区域。这种新的分析程序使得对致密骨精细结构取向进行更详细的研究成为可能，但这有什么意义吗？为了回答这个问题，我们选择了一块体内应变模式已被明确记录的骨头进行特别研究。马桡骨的横向中骨干切片显示CPL亮区的分布与先前报道的应变模式密切相关。
