Centre for Medicine Use and Safety, Faculty of Pharmacy and Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, Victoria 3052, Australia.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Jan 15;913-914:171-5. doi: 10.1016/j.jchromb.2012.11.030. Epub 2012 Dec 20.
The quantification of voriconazole concentration in lung epithelial lining fluid to facilitate the management of pulmonary fungal colonisation or aspergillosis is of increasing interest. An accurate and reproducible high-performance liquid chromatography method to quantify voriconazole in human bronchoalveolar lavage (BAL) fluid was developed and validated. BAL samples were concentrated by freeze-drying and reconstituted with water prior to deproteinisation. Separation was achieved with a C18 column employing fluorescence detection (excitation: 260nm, emission: 370nm). The calibration curves were linear from 2.5 to 500ng/mL. The intra- and inter-day precisions were within 7%. Accuracies ranged from 102% to 107%. The clinical applicability was established by successful measurement of voriconazole concentrations in lung transplant recipients. The assay provides an alternative approach for those with negligible access to liquid chromatography-tandem mass spectrometry instrumentation.
定量检测肺部上皮衬液中的伏立康唑浓度，有助于管理肺部真菌感染或曲霉菌感染，这引起了越来越多的关注。本研究开发并验证了一种准确且可重现的高效液相色谱法，用于检测人支气管肺泡灌洗液（BAL）中的伏立康唑浓度。BAL 样本通过冷冻干燥浓缩，并用去蛋白水复溶。采用 C18 柱，荧光检测（激发波长：260nm，发射波长：370nm）实现分离。校准曲线在 2.5 至 500ng/mL 范围内呈线性。日内和日间精密度均在 7%以内。准确度在 102%至 107%之间。该方法在肺移植受者中成功测量了伏立康唑浓度，证实了其临床适用性。该检测方法为那些几乎无法使用液相色谱-串联质谱仪器的人提供了一种替代方法。
