Department of Diagnostics and Vaccinology, Swedish Institute for Communicable Disease Control, Stockholm, Sweden.
Curr Opin HIV AIDS. 2013 Mar;8(2):87-92. doi: 10.1097/COH.0b013e32835d80af.
This review will describe advances in detection and results of monitoring persistent viremia in patients on long-term suppressive therapy. In addition, the review explores the usefulness of these methods in determining the effectiveness of new HIV-1 eradication strategies in purging persistent HIV-1 reservoirs.
Quantification of plasma HIV-1 RNA levels remains essential for determining the success of combination antiretroviral therapy (cART) in treated patients. Recently, several new platforms with improved sensitivity for quantifying HIV-1 RNA have been developed and the application of these assays has revealed that low-level viremia persists in patients on suppressive therapy. In addition, new technological advances such as digital PCR have been proposed to increase the sensitivity of measuring and characterizing persistent HIV-1 viremia. The application of these assays will be important in determining the effectiveness of future HIV-1 eradication strategies.
The level of HIV-1 RNA in patient plasma remains an important marker for determining the success of cART. New sensitive assays have found that HIV-1 persists in the plasma of patients on suppressive therapy that may have implications for the clinical management of this disease and strategies for eliminating HIV-1 infection.
本综述将描述在长期抑制性治疗患者中持续病毒血症的检测和监测结果的进展。此外，本综述还探讨了这些方法在确定新的 HIV-1 清除策略在清除持续性 HIV-1 储存库方面的有效性方面的有用性。
定量检测血浆 HIV-1 RNA 水平仍然是确定联合抗逆转录病毒治疗 (cART) 在治疗患者中成功的关键。最近，已经开发出几种新的灵敏度更高的定量检测 HIV-1 RNA 的平台，这些检测方法的应用表明，在接受抑制性治疗的患者中，持续存在低水平的病毒血症。此外，还提出了数字 PCR 等新技术进步，以提高测量和描述持续性 HIV-1 病毒血症的灵敏度。这些检测方法的应用对于确定未来 HIV-1 清除策略的有效性非常重要。
患者血浆中的 HIV-1 RNA 水平仍然是确定 cART 成功的重要标志物。新的灵敏检测方法发现，在接受抑制性治疗的患者的血浆中 HIV-1 持续存在，这可能对该疾病的临床管理和消除 HIV-1 感染的策略产生影响。
