Adult Stem Cells Group, Institute of Biomedical Technology, University of Tampere, Tampere, Finland ; BioMediTech, Tampere, Finland ; Science Centre, Tampere University Hospital, Tampere, Finland.
J Tissue Eng. 2012;3(1):2041731412467998. doi: 10.1177/2041731412467998. Epub 2012 Dec 2.
Functional tissue engineering for bone augmentation requires the appropriate combination of biomaterials, mesenchymal stem cells, and specific differentiation factors. Therefore, we investigated the morphology, attachment, viability, and proliferation of human dental pulp stem cells cultured in xeno-free conditions in human serum medium seeded on β-tricalcium phosphate/poly(l-lactic acid/caprolactone) three-dimensional biomaterial scaffold. Additionally, osteogenic inducers dexamethasone and vitamin D(3) were compared to achieve osteogenic differentiation. Dental pulp stem cells cultured in human serum medium maintained their morphology; furthermore, cells attached, remained viable, and increased in cell number within the scaffold. Alkaline phosphatase staining showed the osteogenic potential of dental pulp stem cells under the influence of osteogenic medium containing vitamin D(3) or dexamethasone within the scaffolds. Maintenance of dental pulp stem cells for 14 days in osteogenic medium containing vitamin D(3) resulted in significant increase in osteogenic markers as shown at mRNA level in comparison to osteogenic medium containing dexamethasone. The results of this study show that osteogenic medium containing vitamin D(3) osteo-induced dental pulp stem cells cultured in human serum medium within β-tricalcium phosphate/poly(l-lactic acid/caprolactone) three-dimensional biomaterial, which could be directly translated clinically.
功能性组织工程学需要将生物材料、间充质干细胞和特定分化因子适当结合起来，以实现骨增量。因此，我们研究了在无动物血清条件下，人血清培养基中培养的人牙髓干细胞在β-磷酸三钙/聚（L-乳酸）/己内酯三维生物材料支架上的形态、黏附、活力和增殖情况。此外，我们还比较了成骨诱导剂地塞米松和维生素 D(3)，以实现成骨分化。在人血清培养基中培养的牙髓干细胞保持其形态；此外，细胞在支架内附着、保持活力并增加细胞数量。碱性磷酸酶染色显示，在含有维生素 D(3)或地塞米松的成骨培养基的影响下，支架内的牙髓干细胞具有成骨潜能。与含有地塞米松的成骨培养基相比，在含有维生素 D(3)的成骨培养基中维持牙髓干细胞 14 天，在 mRNA 水平上显著增加了成骨标志物的表达。本研究结果表明，在β-磷酸三钙/聚（L-乳酸）/己内酯三维生物材料中，含有维生素 D(3)的成骨培养基可诱导人血清培养基中培养的牙髓干细胞，这一结果可直接转化为临床应用。
