Zargaran Massoumeh, Eshghyar Nosratollah, Baghaei Fahimeh, Moghimbeigi Abbas
Dental Research Center, Department of Oral and Maxillofacial Pathology, Dental School, Hamadan University of Medical Sciences, Hamadan, Iran.
Asian Pac J Cancer Prev. 2012;13(11):5811-5. doi: 10.7314/apjcp.2012.13.11.5811.
Non-invasive oral verrucous carcinoma (OVC) and invasive well-differentiated oral squamous cell carcinoma (OSCC) have similar histopathologic findings but different biological behavior. These two malignancies must be correctly differentiated by pathologists. The aim of this study was to determine immunohistochemical (IHC) expression of Ki67 in OVC and well-differentiated OSCC.
Expression of Ki67 was evaluated by IHC in 15 cases of epithelial hyperplasia with no dysplasia (A group), 15 cases of OVC (B group), 12 cases of microinvasive OSCC(C group) and 15 cases of well-differentiated OSCC (D group).
There was a significant difference in Ki67 expression based on pattern distribution of immunostaining positive cells, with quantitative and semi-quantitative analyses, among the four groups ; also, between A group and each of the other three groups (P=0.0001). But there was no significant difference between B and C, C and D, and B and D groups (P>0.05).
The three evaluation methods of Ki67 expression showed Ki67 (Mib-1) is not a good immunohistochemical marker to assess invasion status and differentiate OVC from well-differentiated OSCC; also, it cannot be used as a diagnostic tool to distinguish between variants of OSCC with similar grade.
非侵袭性口腔疣状癌（OVC）和侵袭性高分化口腔鳞状细胞癌（OSCC）具有相似的组织病理学表现，但生物学行为不同。这两种恶性肿瘤必须由病理学家正确区分。本研究的目的是确定Ki67在OVC和高分化OSCC中的免疫组化（IHC）表达。
通过免疫组化评估15例无发育异常的上皮增生（A组）、15例OVC（B组）、12例微侵袭性OSCC（C组）和15例高分化OSCC（D组）中Ki67的表达。
基于免疫染色阳性细胞的模式分布，通过定量和半定量分析，四组之间Ki67表达存在显著差异；A组与其他三组之间也存在显著差异（P = 0.0001）。但B组与C组、C组与D组以及B组与D组之间无显著差异（P>0.05）。
Ki67表达的三种评估方法表明，Ki67（Mib-1）不是评估侵袭状态以及区分OVC和高分化OSCC的良好免疫组化标志物；此外，它不能用作区分相似分级的OSCC变体的诊断工具。
