• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

改良高蔗糖饮食诱导的腹部肥胖和正常体重大鼠表现出高血浆游离脂肪酸和胰岛素抵抗。

Modified high-sucrose diet-induced abdominally obese and normal-weight rats developed high plasma free fatty acid and insulin resistance.

机构信息

Division of Geriatrics, West China Hospital of Sichuan University, Chengdu 610041, China.

出版信息

Oxid Med Cell Longev. 2012;2012:374346. doi: 10.1155/2012/374346. Epub 2012 Dec 25.

DOI:10.1155/2012/374346
PMID:23320128
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3540965/
Abstract

INTRODUCTION

Metabolically obese but normal-weight (MONW) individuals have metabolic features of overt obesity, and abdominal adiposity is common in them. Animal models of MONW individuals are lacking. We aimed to develop an abdominally obese and normal-weight (AONW) rat model.

METHODS AND RESULTS

Young male Sprague-Dawley rats were fed chow or a modified high-sucrose (HS) diet for 20 weeks. The HS diet induced increased visceral adipose tissue without increased body weight, reduced glucose disposal rates, and increased hepatic glucose output during the hyperinsulinemic-euglycemic clamp, increased plasma glucose during the intraperitoneal glucose tolerance test, and increased plasma free fatty acids. Hepatic lipidosis and hepatocyte mitochondria swelling were found in HS rats through light microscopy and transmission electron microscopy; similar impairments were not observed in muscle. RT-PCR showed that mRNA expression of uncoupling protein 3 and peroxisome proliferator-activated receptor-gamma coactivator 1α increased in muscle of HS rats, while expression of mitochondrial transcription factor A, glucose transporter type 4, and insulin receptor substrate-1 did not change significantly.

CONCLUSION

AONW rats developed metabolic disorders seen in MONW individuals. Steatosis, mitochondrial morphologic changes, and insulin resistance were more serious in liver than in muscle. Genes involved in fatty acid metabolism and mitochondrial function changed in less impaired muscle.

摘要

简介

代谢肥胖但体重正常（MONW）个体具有明显肥胖的代谢特征，并且腹部肥胖在他们中很常见。MONW 个体的动物模型缺乏。我们旨在开发一种腹部肥胖和体重正常（AONW）大鼠模型。

方法和结果

年轻雄性 Sprague-Dawley 大鼠喂食标准饲料或改良高蔗糖（HS）饮食 20 周。HS 饮食诱导内脏脂肪组织增加而体重增加，葡萄糖处置率降低，在高胰岛素-正常血糖钳夹期间肝葡萄糖输出增加，腹腔内葡萄糖耐量试验期间血浆葡萄糖增加，游离脂肪酸增加。通过光镜和透射电镜观察到 HS 大鼠存在肝脂肪变性和肝细胞线粒体肿胀；在肌肉中未观察到类似的损伤。RT-PCR 显示 HS 大鼠肌肉中解偶联蛋白 3 和过氧化物酶体增殖物激活受体-γ共激活因子 1α 的 mRNA 表达增加，而线粒体转录因子 A、葡萄糖转运蛋白 4 和胰岛素受体底物-1 的表达没有明显变化。

结论

AONW 大鼠出现了 MONW 个体中出现的代谢紊乱。肝内脂肪变性、线粒体形态变化和胰岛素抵抗比肌肉更严重。在受损较轻的肌肉中，参与脂肪酸代谢和线粒体功能的基因发生了变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/ae53aa48e282/OXIMED2012-374346.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/810e59af4533/OXIMED2012-374346.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/adbc2e1bec58/OXIMED2012-374346.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/0a943606fd72/OXIMED2012-374346.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/f1df6716c6ee/OXIMED2012-374346.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/b79127a66650/OXIMED2012-374346.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/3734488fa779/OXIMED2012-374346.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/6d14f48aa2af/OXIMED2012-374346.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/ae53aa48e282/OXIMED2012-374346.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/810e59af4533/OXIMED2012-374346.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/adbc2e1bec58/OXIMED2012-374346.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/0a943606fd72/OXIMED2012-374346.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/f1df6716c6ee/OXIMED2012-374346.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/b79127a66650/OXIMED2012-374346.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/3734488fa779/OXIMED2012-374346.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/6d14f48aa2af/OXIMED2012-374346.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d8a8/3540965/ae53aa48e282/OXIMED2012-374346.008.jpg

相似文献

1
Modified high-sucrose diet-induced abdominally obese and normal-weight rats developed high plasma free fatty acid and insulin resistance.改良高蔗糖饮食诱导的腹部肥胖和正常体重大鼠表现出高血浆游离脂肪酸和胰岛素抵抗。
Oxid Med Cell Longev. 2012;2012:374346. doi: 10.1155/2012/374346. Epub 2012 Dec 25.
2
Ping-tang Recipe () improves insulin resistance and attenuates hepatic steatosis in high-fat diet-induced obese rats.平塘方（）改善高脂饮食诱导肥胖大鼠的胰岛素抵抗和肝脂肪变性。
Chin J Integr Med. 2012 Apr;18(4):262-8. doi: 10.1007/s11655-012-1023-0. Epub 2012 Mar 30.
3
Effects of dietary phosphate on glucose and lipid metabolism.膳食磷酸盐对糖和脂代谢的影响。
Am J Physiol Endocrinol Metab. 2016 Apr 1;310(7):E526-38. doi: 10.1152/ajpendo.00234.2015. Epub 2016 Jan 19.
4
Dietary capsaicin reduces obesity-induced insulin resistance and hepatic steatosis in obese mice fed a high-fat diet.膳食辣椒素可降低高脂肪饮食诱导肥胖小鼠的肥胖诱导性胰岛素抵抗和肝脂肪变性。
Obesity (Silver Spring). 2010 Apr;18(4):780-7. doi: 10.1038/oby.2009.301. Epub 2009 Oct 1.
5
Leucine improves glucose and lipid status in offspring from obese dams, dependent on diet type, but not caloric intake.亮氨酸可改善肥胖母鼠后代的葡萄糖和脂质状况，这取决于饮食类型，而不是热量摄入。
J Neuroendocrinol. 2012 Oct;24(10):1356-64. doi: 10.1111/j.1365-2826.2012.02339.x.
6
Metabolic implications of dietary trans-fatty acids.膳食反式脂肪酸的代谢影响
Obesity (Silver Spring). 2009 Jun;17(6):1200-7. doi: 10.1038/oby.2008.662. Epub 2009 Feb 19.
7
Jueming Prescription reduces body weight by increasing the mRNA expressions of beta3-adrenergic receptor and uncoupling protein-2 in adipose tissue of diet-induced obese rats.决明降脂方通过增加饮食诱导肥胖大鼠脂肪组织中β3-肾上腺素能受体和解偶联蛋白-2 的 mRNA 表达来降低体重。
Chin J Integr Med. 2012 Oct;18(10):775-81. doi: 10.1007/s11655-011-0959-9. Epub 2012 Mar 28.
8
Enzymatically synthesized glycogen reduces lipid accumulation in diet-induced obese rats.酶合成的糖原可减少饮食诱导肥胖大鼠的脂肪积累。
Nutr Res. 2013 Sep;33(9):743-52. doi: 10.1016/j.nutres.2013.06.006. Epub 2013 Aug 1.
9
Pioglitazone can ameliorate insulin resistance in low-dose streptozotocin and high sucrose-fat diet induced obese rats.吡格列酮可改善低剂量链脲佐菌素和高蔗糖-脂肪饮食诱导的肥胖大鼠的胰岛素抵抗。
Acta Pharmacol Sin. 2005 May;26(5):575-80. doi: 10.1111/j.1745-7254.2005.00090.x.
10
The Lou/C rat: a model of spontaneous food restriction associated with improved insulin sensitivity and decreased lipid storage in adipose tissue.Lou/C大鼠：一种与胰岛素敏感性改善及脂肪组织脂质储存减少相关的自发性食物限制模型。
Am J Physiol Endocrinol Metab. 2009 May;296(5):E1120-32. doi: 10.1152/ajpendo.90592.2008. Epub 2009 Feb 10.

引用本文的文献

1
High-Caloric Diets in Adolescence Impair Specific GABAergic Subpopulations, Neurogenesis, and Alter Astrocyte Morphology.青春期高卡路里饮食会损害特定的 GABA 能亚群、神经发生和改变星形胶质细胞形态。
Int J Mol Sci. 2024 May 18;25(10):5524. doi: 10.3390/ijms25105524.
2
The Effect of Alternating High-Sucrose and Sucrose Free-Diets, and Intermittent One-Day Fasting on the Estrous Cycle and Sex Hormones in Female Rats.交替高糖和无糖饮食以及间歇性一天禁食对雌性大鼠动情周期和性激素的影响。
Nutrients. 2022 Oct 17;14(20):4350. doi: 10.3390/nu14204350.
3
Carob Extract Supplementation Together with Caloric Restriction and Aerobic Training Accelerates the Recovery of Cardiometabolic Health in Mice with Metabolic Syndrome.

本文引用的文献

1
PGC-1α induces mitochondrial and myokine transcriptional programs and lipid droplet and glycogen accumulation in cultured human skeletal muscle cells.PGC-1α 诱导培养的人骨骼肌细胞中线粒体和肌肉因子的转录程序以及脂滴和糖原的积累。
PLoS One. 2012;7(1):e29985. doi: 10.1371/journal.pone.0029985. Epub 2012 Jan 17.
2
Significance of uncoupling protein 3 in mitochondrial function upon mid- and long-term dietary high-fat exposure.解偶联蛋白 3 在中期和长期高脂肪饮食暴露下对线粒体功能的意义。
FEBS Lett. 2011 Dec 15;585(24):4010-7. doi: 10.1016/j.febslet.2011.11.012. Epub 2011 Nov 21.
3
Overexpression of PGC-1α increases fatty acid oxidative capacity of human skeletal muscle cells.
角豆提取物补充剂与热量限制和有氧运动训练相结合可加速代谢综合征小鼠心脏代谢健康的恢复。
Antioxidants (Basel). 2022 Sep 13;11(9):1803. doi: 10.3390/antiox11091803.
4
Metabolic Syndrome: Lessons from Rodent and Models.代谢综合征：啮齿动物模型的启示。
Biomed Res Int. 2022 Jun 22;2022:5850507. doi: 10.1155/2022/5850507. eCollection 2022.
5
Role of Ventromedial Hypothalamus in Sucrose-Induced Obesity on Metabolic Parameters.腹内侧下丘脑在蔗糖诱导的肥胖对代谢参数影响中的作用。
Ann Neurosci. 2021 Jan;28(1-2):39-46. doi: 10.1177/09727531211005738. Epub 2021 May 21.
6
Hippocampal electrophysiological changes during the elicited metabolic syndrome in Wistar rats.Wistar大鼠诱发代谢综合征期间海马的电生理变化
Metabol Open. 2020 Feb 7;5:100027. doi: 10.1016/j.metop.2020.100027. eCollection 2020 Mar.
7
A systematic review on different models of inducing obesity in animals: Advantages and limitations.关于动物诱导肥胖不同模型的系统评价：优势与局限性
J Adv Vet Anim Res. 2019 Dec 14;7(1):103-114. doi: 10.5455/javar.2020.g399. eCollection 2020 Mar.
8
Differential Effects of High Sugar, High Lard or a Combination of Both on Nutritional, Hormonal and Cardiovascular Metabolic Profiles of Rodents.高糖、高猪油或两者组合对啮齿动物营养、激素和心血管代谢特征的差异影响。
Nutrients. 2018 Aug 11;10(8):1071. doi: 10.3390/nu10081071.
9
Maternal consumption of a cafeteria diet during lactation in rats leads the offspring to a thin-outside-fat-inside phenotype.大鼠哺乳期母体食用自助餐式饮食会导致其后代出现外瘦内胖的表型。
Int J Obes (Lond). 2017 Aug;41(8):1279-1287. doi: 10.1038/ijo.2017.42. Epub 2017 Feb 13.
10
High-fat but not sucrose intake is essential for induction of dyslipidemia and non-alcoholic steatohepatitis in guinea pigs.高脂肪而非高糖摄入对诱导豚鼠血脂异常和非酒精性脂肪性肝炎至关重要。
Nutr Metab (Lond). 2016 Aug 9;13:51. doi: 10.1186/s12986-016-0110-1. eCollection 2016.
过表达PGC-1α可提高人类骨骼肌细胞的脂肪酸氧化能力。
Biochem Res Int. 2012;2012:714074. doi: 10.1155/2012/714074. Epub 2011 Sep 4.
4
Time-resolved and tissue-specific systems analysis of the pathogenesis of insulin resistance.胰岛素抵抗发病机制的时分辨和组织特异性系统分析。
PLoS One. 2010 Jan 21;5(1):e8817. doi: 10.1371/journal.pone.0008817.
5
Long-term high-fat feeding induces greater fat storage in mice lacking UCP3.长期高脂喂养会导致缺乏解偶联蛋白3（UCP3）的小鼠储存更多脂肪。
Am J Physiol Endocrinol Metab. 2008 Nov;295(5):E1018-24. doi: 10.1152/ajpendo.00779.2007. Epub 2008 Aug 19.
6
Alterations in hepatic mitochondrial compartment in a model of obesity and insulin resistance.肥胖和胰岛素抵抗模型中肝脏线粒体区室的改变。
Obesity (Silver Spring). 2008 May;16(5):958-64. doi: 10.1038/oby.2008.10. Epub 2008 Feb 14.
7
Modest PGC-1alpha overexpression in muscle in vivo is sufficient to increase insulin sensitivity and palmitate oxidation in subsarcolemmal, not intermyofibrillar, mitochondria.在体内肌肉中适度过表达PGC-1α足以增加肌膜下而非肌原纤维间线粒体的胰岛素敏感性和棕榈酸氧化。
J Biol Chem. 2008 Feb 15;283(7):4228-40. doi: 10.1074/jbc.M704332200. Epub 2007 Dec 12.
8
High-fat diet impairs the effects of a single bout of endurance exercise on glucose transport and insulin sensitivity in rat skeletal muscle.高脂饮食会削弱单次耐力运动对大鼠骨骼肌葡萄糖转运和胰岛素敏感性的影响。
Metabolism. 2007 Dec;56(12):1719-28. doi: 10.1016/j.metabol.2007.07.017.
9
Overexpression of uncoupling protein 3 in skeletal muscle protects against fat-induced insulin resistance.骨骼肌中解偶联蛋白3的过表达可预防脂肪诱导的胰岛素抵抗。
J Clin Invest. 2007 Jul;117(7):1995-2003. doi: 10.1172/JCI13579.
10
Paradoxical changes in muscle gene expression in insulin-resistant subjects after sustained reduction in plasma free fatty acid concentration.血浆游离脂肪酸浓度持续降低后，胰岛素抵抗受试者肌肉基因表达的反常变化。
Diabetes. 2007 Mar;56(3):743-52. doi: 10.2337/db06-0840.