Varga M, Buris L
College of Pharmacy, Center for Drug Design and Delivery, J. Hillis Miller Health Center, Gainesville, FL 32610.
Pharmacol Biochem Behav. 1989 Jul;33(3):523-6. doi: 10.1016/0091-3057(89)90380-8.
The (+)-cyanidanol-3 is used as an antihepatotoxic and hepatoprotective drug in both men and animals against alcoholic and experimental liver injury. Histologic staining techniques give mostly qualitative or semiquantitative description of liver damages. Experiments have been carried out to determine the hepatoprotective effects of (+)-cyanidanol-3 on alcoholic liver damage (i.e., fatty liver and hepatomegaly) by morphometric measurement of the liver tissue sections. Ethanol was administered ad lib to CFY rats to cause mild alcoholic liver damage together with 200 mg/kg/day (+)-cyanidanol-3 to prevent the tissue deterioration. The changes of hepatic lobule and hepatocytes were measured morphometrically. The chronic ethanol consumption results in hepatocellular hypertrophy, a significant increase in size of the hepatocytes and a mild increase of the intralobular extrahepatocytic space as well when compared with controls. The volume of cytoplasm was increased while the parameters of nuclei were unchanged. The (+)-cyanidanol-3 prevents changes and the morphometric parameters in the treated group were almost the same as in the controls. The treatment with (+)-cyanidanol-3 alone does not affect the hepatic tissue parameters. The results show the hepatoprotective effect of (+)-cyanidanol-3 and the suitability of the morphometric method for quantitative comparison of normal and experimentally-altered liver cells.
（+）-矢车菊素-3在人和动物中均被用作抗肝毒性和肝脏保护药物，用于对抗酒精性和实验性肝损伤。组织学染色技术大多只能对肝损伤进行定性或半定量描述。已经开展实验，通过对肝组织切片进行形态计量学测量，来确定（+）-矢车菊素-3对酒精性肝损伤（即脂肪肝和肝肿大）的肝脏保护作用。对CFY大鼠随意给予乙醇以造成轻度酒精性肝损伤，同时给予200毫克/千克/天的（+）-矢车菊素-3以防止组织恶化。通过形态计量学测量肝小叶和肝细胞的变化。与对照组相比，长期摄入乙醇会导致肝细胞肥大，肝细胞大小显著增加，小叶内肝外细胞间隙也略有增加。细胞质体积增加，而细胞核参数未变。（+）-矢车菊素-3可防止这些变化，治疗组的形态计量学参数与对照组几乎相同。单独使用（+）-矢车菊素-3进行治疗不会影响肝脏组织参数。结果表明了（+）-矢车菊素-3的肝脏保护作用以及形态计量学方法用于正常和实验性改变的肝细胞定量比较的适用性。
