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通过多萜醇中间体在线粒体、富含高尔基体的组分和富含内质网的组分中进行N-糖蛋白合成的比较研究。

Comparative study of the N-glycoprotein synthesis through dolichol intermediates in mitochondria, Golgi apparatus-rich fraction and endoplasmic reticulum-rich fraction.

作者信息

Levrat C, Ardail D, Louisot P

机构信息

Department of Biochemistry, University of Lyon, INSERM-CNRS U. 189, Lyon-Sud Medical School, Oullins, France.

出版信息

Int J Biochem. 1990;22(3):287-93. doi: 10.1016/0020-711x(90)90342-z.

DOI:10.1016/0020-711x(90)90342-z
PMID:2332109
Abstract
  1. Glycosylation of endogenous dolichol acceptors was higher in mitochondria than in C 30,000 g (Golgi apparatus-rich fraction) and C 100,000 g (endoplasmic reticulum-rich fraction). 2. In mitochondria, N-glycoprotein biosynthesized were composed of high mannose type and non-fucosylated biantennary complex type while in C 30,000 g and C 100,000 g preparations, they contained biantennary complex type as tri and tetraantennary complex type oligosaccharides in both fucosylated and non-fucosylated forms.
摘要
  1. 内源性多萜醇受体的糖基化在线粒体中比在30,000g组分(富含高尔基体的部分)和100,000g组分(富含内质网的部分)中更高。2. 在线粒体中,生物合成的N-糖蛋白由高甘露糖型和非岩藻糖基化的双天线复合型组成,而在30,000g和100,000g组分中,它们含有岩藻糖基化和非岩藻糖基化形式的三天线和四天线复合型寡糖的双天线复合型。

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Mol Cell Biochem. 1997 Oct;175(1-2):71-80. doi: 10.1023/a:1006889328983.
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Role of environmental conditions on the expression levels, glycoform pattern and levels of sialyltransferase for hFSH produced by recombinant CHO cells.环境条件对重组CHO细胞产生的人促卵泡激素(hFSH)的表达水平、糖型模式和唾液酸转移酶水平的作用。
Cytotechnology. 1994;15(1-3):217-21. doi: 10.1007/BF00762396.