Research Unit Mycotoxines, Phycotoxines and Associated Pathologies, Faculty of Pharmacy, Monastir 5000, Tunisia.
Ecotoxicol Environ Saf. 2013 Mar;89:1-7. doi: 10.1016/j.ecoenv.2012.12.015. Epub 2013 Jan 12.
Cadmium (Cd) is a non-essential metal which could be toxic in traces for aquatic species. Increasing Cd concentrations in sea water is mainly related to waste waters provided from growing industrial and agricultural activities. The present study investigated the accumulation of Cd (0.5mg/L) in different tissues of Sparus aurata under a short term exposure (2, 4 and 24h) using the atomic absorption. This work investigated also the impact of the metal on oxidative stress biomarkers and acetyl cholinesterase activity (AchE). Our results showed that Cd accumulation in different tissues depends largely on the length of the exposure period to the metal. Indeed, the highest concentrations were obtained after 24h. Cd accumulation in tissues was in the following order: intestines > liver > gills > dorsal muscle. Cadmium administration increased significantly catalase activity (CAT), glutathione level (GSH) and malondialdehyde production (MDA) after 24h of exposure. In contrast, AchE activity was decreased after the same period of exposure to the metal. There were no significant changes in oxidative stress biomarkers after 2 and 4h of exposure, except for superoxide dismutase (SOD) activity which attained the highest level after 4h. These results suggest that short-term exposure of Sparus aurata to Cd (0.5mg/L) induced an important metal accumulation in intestine and a notable oxidative stress response.
镉（Cd）是一种非必需的金属，在痕量下对水生生物可能有毒。海水中镉浓度的增加主要与工业和农业活动日益增长的废水有关。本研究采用原子吸收法研究了短时间暴露（2、4 和 24 小时）下， Cd（0.5mg/L）在真鲷不同组织中的积累情况。本研究还研究了金属对氧化应激生物标志物和乙酰胆碱酯酶活性（AchE）的影响。我们的研究结果表明，Cd 在不同组织中的积累在很大程度上取决于暴露于金属的时间长短。实际上，24 小时后获得的浓度最高。Cd 在组织中的积累顺序为：肠道>肝脏>鳃>背肌。暴露于 Cd 24 小时后，CAT 活性、GSH 水平和 MDA 产量显著增加。相反，在暴露于金属的相同时期，AchE 活性下降。暴露 2 和 4 小时后，除超氧化物歧化酶（SOD）活性在 4 小时后达到最高水平外，其他氧化应激生物标志物没有显著变化。这些结果表明，真鲷短期暴露于 Cd（0.5mg/L）会导致肠道中大量的金属积累和明显的氧化应激反应。
